Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum

Celiac Disease (CD) is an interferon (IFN)γ-mediated duodenal hypersensitivity to wheat gluten occurring in genetically predisposed individuals. Gluten-free diet (GFD) leads to a complete remission of the disease. Vα24-restricted invariant NKT (iNKT) cells are important to maintain immune homeostasi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Montalvillo, Enrique, Bernardo, David, Martínez Abad, Beatriz, Allegretti, Yessica Lorena, Fernández Salazar, Luis, Calvo, Carmen, Chirdo, Fernando Gabriel, Garrote, José A., Arranz, Eduardo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2015
Materias:	Ciencias Exactas Celiac disease Celiac disease-like molecular profile IFNγ INKT Intraepithelial lymphocytes Vα24-Jα18
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/86366
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-86366
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Celiac disease Celiac disease-like molecular profile IFNγ INKT Intraepithelial lymphocytes Vα24-Jα18
spellingShingle	Ciencias Exactas Celiac disease Celiac disease-like molecular profile IFNγ INKT Intraepithelial lymphocytes Vα24-Jα18 Montalvillo, Enrique Bernardo, David Martínez Abad, Beatriz Allegretti, Yessica Lorena Fernández Salazar, Luis Calvo, Carmen Chirdo, Fernando Gabriel Garrote, José A. Arranz, Eduardo Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
topic_facet	Ciencias Exactas Celiac disease Celiac disease-like molecular profile IFNγ INKT Intraepithelial lymphocytes Vα24-Jα18
description	Celiac Disease (CD) is an interferon (IFN)γ-mediated duodenal hypersensitivity to wheat gluten occurring in genetically predisposed individuals. Gluten-free diet (GFD) leads to a complete remission of the disease. Vα24-restricted invariant NKT (iNKT) cells are important to maintain immune homeostasis in the gut mucosa because of their unique capacity to rapidly produce large quantities of both T-helper (Th)1 and Th2 cytokines upon stimulation. We studied the presence of these cells in the CD duodenum. Duodenal biopsies were obtained from 45 untreated-CD patients (uCD), 15 Gluten Free Diet-CD patients (GFD-CD), 44 non-inflamed non-CD controls (C-controls) and 15 inflamed non-CD controls (I-controls). Two populations from Spain and Argentina were recruited. Messenger RNA (mRNA) expression of Vα24-Jα18 (<i>invariant</i> TCRα chain of human iNKT cells), IFNγ and intracellular transcription factor Forkhead Box P3 (Foxp3), and flow cytometry intraepithelial lymphocyte (IEL) profile were determined. Both uCD and GFD-CD patients had higher Vα24-Jα18 mRNA levels than non-CD controls (I and C-controls). The expression of Vα24-Jα18 correlated with Marsh score for the severity of mucosal lesion and also with increased mRNA IFNγ levels. uCD and GFD-CD patients had decreased mRNA expression of FoxP3 but increased expression of Vα24-Jα18, which revealed a CD-like molecular profile. Increased numbers of iNKT cells were confirmed by flow cytometry within the intraepithelial lymphocyte compartment of uCD and GFD-CD patients and correlated with Vα24-Jα18 mRNA expression. In conclusion, we have found an increased number of iNKT cells in the duodenum from both uCD and GFD-CD patients, irrespective of the mucosal status. A CD-like molecular profile, defined by an increased mRNA expression of Vα24-Jα18 together with a decreased expression of FoxP3, may represent a pro-inflammatory signature of the CD duodenum.
format	Articulo Articulo
author	Montalvillo, Enrique Bernardo, David Martínez Abad, Beatriz Allegretti, Yessica Lorena Fernández Salazar, Luis Calvo, Carmen Chirdo, Fernando Gabriel Garrote, José A. Arranz, Eduardo
author_facet	Montalvillo, Enrique Bernardo, David Martínez Abad, Beatriz Allegretti, Yessica Lorena Fernández Salazar, Luis Calvo, Carmen Chirdo, Fernando Gabriel Garrote, José A. Arranz, Eduardo
author_sort	Montalvillo, Enrique
title	Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title_short	Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title_full	Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title_fullStr	Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title_full_unstemmed	Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum
title_sort	increased intraepithelial vα24 invariant nkt cells in the celiac duodenum
publishDate	2015
url	http://sedici.unlp.edu.ar/handle/10915/86366
work_keys_str_mv	AT montalvilloenrique increasedintraepithelialva24invariantnktcellsintheceliacduodenum AT bernardodavid increasedintraepithelialva24invariantnktcellsintheceliacduodenum AT martinezabadbeatriz increasedintraepithelialva24invariantnktcellsintheceliacduodenum AT allegrettiyessicalorena increasedintraepithelialva24invariantnktcellsintheceliacduodenum AT fernandezsalazarluis increasedintraepithelialva24invariantnktcellsintheceliacduodenum AT calvocarmen increasedintraepithelialva24invariantnktcellsintheceliacduodenum AT chirdofernandogabriel increasedintraepithelialva24invariantnktcellsintheceliacduodenum AT garrotejosea increasedintraepithelialva24invariantnktcellsintheceliacduodenum AT arranzeduardo increasedintraepithelialva24invariantnktcellsintheceliacduodenum
bdutipo_str	Repositorios
_version_	1764820489615704066

Increased intraepithelial Vα24 invariant NKT cells in the celiac duodenum

Ejemplares similares