Histochemical differences along the intestine of <i>Corydoras paleatus</i> (Siluriformes: Callichthyidae)

The Neotropical catfish Corydoras paleatus is a facultative air-breather and the caudal half of the intestine is involved in gas exchange. In South America, air-breathing fishes are found in tropical or sub-tropical freshwaters where the probability of hypoxia is high. The aim of this study was to c...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Plaul, Silvia Elena, Barbeito, Claudio Gustavo, Díaz, Alcira Ofelia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Veterinarias
Corydoras paleatus
Glycoconjugates
Goblet cells
Histochemistry
Intestine
Lectinhistochemistry
Glicoconjugados
Histoquímica
Intestinos
Lectinhistoquímica
Células Caliciformes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86348
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86348
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Corydoras paleatus
Glycoconjugates
Goblet cells
Histochemistry
Intestine
Lectinhistochemistry
Glicoconjugados
Histoquímica
Intestinos
Lectinhistoquímica
Células Caliciformes
spellingShingle Ciencias Veterinarias
Corydoras paleatus
Glycoconjugates
Goblet cells
Histochemistry
Intestine
Lectinhistochemistry
Glicoconjugados
Histoquímica
Intestinos
Lectinhistoquímica
Células Caliciformes
Plaul, Silvia Elena
Barbeito, Claudio Gustavo
Díaz, Alcira Ofelia
Histochemical differences along the intestine of <i>Corydoras paleatus</i> (Siluriformes: Callichthyidae)
topic_facet Ciencias Veterinarias
Corydoras paleatus
Glycoconjugates
Goblet cells
Histochemistry
Intestine
Lectinhistochemistry
Glicoconjugados
Histoquímica
Intestinos
Lectinhistoquímica
Células Caliciformes
description The Neotropical catfish Corydoras paleatus is a facultative air-breather and the caudal half of the intestine is involved in gas exchange. In South America, air-breathing fishes are found in tropical or sub-tropical freshwaters where the probability of hypoxia is high. The aim of this study was to characterize by traditional histochemical and lectinhistochemical methods the pattern of carbohydrate in the intestinal mucosa. Intestine samples were taken from 25 healthy adult specimens collected in Buenos Aires (Argentina). Samples were fixed by immersion in 10 % buffered formalin and routinely processed and embedded in paraffin wax. Subsequently, these sections were incubated in the biotinylated lectins battery. Labeled Streptavidin-Biotin (LSAB) system was used for detection, diaminobenzidine as chromogen and haematoxylin as a contrast. To locate and distinguish glycoconjugates (GCs) of the globet cells, we used the following histochemical methods: PAS; PAS*S; KOH/ PA*S; PA/Bh/KOH/PAS; KOH/PA*/Bh/PAS; Alcian Blue and Toluidine Blue at different pHs. Microscopically, the general structure of vertebrate intestine was observed and showed all the cell types characteristic of the intestinal epithelium. The cranial sector of catfish intestine is a site of digestion and absorption and its structure is similar to other fish groups. In contrast, enterocytes of the caudal portion are low cuboidal cells; and between these, globet cells and capillaries are observed, these latter may reach the mucosal lumen. Underlying the epithelium, observed a well-developed lamina propria-submucosa made of connective tissue; this layer was highly vascularized and did not exhibit glands. According to histochemistry, the diverse GCs elaborated and secreted in the intestine are associated with specific functions in relation to their physiological significance, with special reference to their role in lubrication, buffering effect and prevention of proteolytic damage to the epithelium together with other biological processes, such as osmoregulation and ion exchange. The lectinhistochemical analysis of the intestinal mucosa reveals the presence of terminal residues of glucose, mannose and galactose. In conclusion, this study has shown that GCs synthesized in the intestine of C. paleatus exhibit a high level of histochemical complexity and that the lectin binding pattern of the intestinal mucosa is characteristic of each species and the variations are related with the multiple functions performed by the mucus in the digestive tract. The information generated here may be a relevant biological tool for comparing and analyzing the possible glycosidic changes in the intestinal mucus under different conditions, such as changes in diet or different pathological stages.
format Articulo
Articulo
author Plaul, Silvia Elena
Barbeito, Claudio Gustavo
Díaz, Alcira Ofelia
author_facet Plaul, Silvia Elena
Barbeito, Claudio Gustavo
Díaz, Alcira Ofelia
author_sort Plaul, Silvia Elena
title Histochemical differences along the intestine of <i>Corydoras paleatus</i> (Siluriformes: Callichthyidae)
title_short Histochemical differences along the intestine of <i>Corydoras paleatus</i> (Siluriformes: Callichthyidae)
title_full Histochemical differences along the intestine of <i>Corydoras paleatus</i> (Siluriformes: Callichthyidae)
title_fullStr Histochemical differences along the intestine of <i>Corydoras paleatus</i> (Siluriformes: Callichthyidae)
title_full_unstemmed Histochemical differences along the intestine of <i>Corydoras paleatus</i> (Siluriformes: Callichthyidae)
title_sort histochemical differences along the intestine of <i>corydoras paleatus</i> (siluriformes: callichthyidae)
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/86348
work_keys_str_mv AT plaulsilviaelena histochemicaldifferencesalongtheintestineoficorydoraspaleatusisiluriformescallichthyidae
AT barbeitoclaudiogustavo histochemicaldifferencesalongtheintestineoficorydoraspaleatusisiluriformescallichthyidae
AT diazalciraofelia histochemicaldifferencesalongtheintestineoficorydoraspaleatusisiluriformescallichthyidae
AT plaulsilviaelena diferenciashistoquimicasalolargodelintestinodecorydoraspaleatussiluriformescallichthyidae
AT barbeitoclaudiogustavo diferenciashistoquimicasalolargodelintestinodecorydoraspaleatussiluriformescallichthyidae
AT diazalciraofelia diferenciashistoquimicasalolargodelintestinodecorydoraspaleatussiluriformescallichthyidae
bdutipo_str Repositorios
_version_ 1764820489596829698

Ejemplares similares