Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin

Oxidized pterins, efficient photosensitizers under UVA irradiation, accumulate in the skin of patients suffering from vitiligo, a chronic depigmentation disorder. Soybean phosphatidylcholine (SoyPC) liposomes were employed as model membranes to investigate if pterin (Ptr), the parent compound of oxi...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Thomas, Andrés Héctor, Catalá, Ángel, Vignoni, Mariana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Química
Física
Hydroperoxide
Liposome
Phosphatidylcholine
Pterin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86070
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86070
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Física
Hydroperoxide
Liposome
Phosphatidylcholine
Pterin
spellingShingle Química
Física
Hydroperoxide
Liposome
Phosphatidylcholine
Pterin
Thomas, Andrés Héctor
Catalá, Ángel
Vignoni, Mariana
Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
topic_facet Química
Física
Hydroperoxide
Liposome
Phosphatidylcholine
Pterin
description Oxidized pterins, efficient photosensitizers under UVA irradiation, accumulate in the skin of patients suffering from vitiligo, a chronic depigmentation disorder. Soybean phosphatidylcholine (SoyPC) liposomes were employed as model membranes to investigate if pterin (Ptr), the parent compound of oxidized pterins, is able to photoinduced lipid peroxidation. Size exclusion chromatography and dialysis experiments showed that Ptr is not encapsulated inside the liposomes and the lipid membrane is permeable to this compound. The formation of conjugated dienes and trienes, upon UVA irradiation, was followed by absorption at 234 and 270 nm, respectively. The photoproducts were characterized by mass spectrometry and oxygenation of SoyPC was demonstrated. In addition, analysis of MS/MS spectra suggested the formation hydroperoxides. Finally, the biological implications of the findings are discussed.
format Articulo
Articulo
author Thomas, Andrés Héctor
Catalá, Ángel
Vignoni, Mariana
author_facet Thomas, Andrés Héctor
Catalá, Ángel
Vignoni, Mariana
author_sort Thomas, Andrés Héctor
title Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title_short Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title_full Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title_fullStr Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title_full_unstemmed Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title_sort soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/86070
work_keys_str_mv AT thomasandreshector soybeanphosphatidylcholineliposomesasmodelmembranestostudylipidperoxidationphotoinducedbypterin
AT catalaangel soybeanphosphatidylcholineliposomesasmodelmembranestostudylipidperoxidationphotoinducedbypterin
AT vignonimariana soybeanphosphatidylcholineliposomesasmodelmembranestostudylipidperoxidationphotoinducedbypterin
bdutipo_str Repositorios
_version_ 1764820489263382531

Ejemplares similares