In Vivo and in Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

The aim of this work was to assess the biological and food safety of two different beverages: Classic Coca Cola™ (CCC) and Caffeine-Free Coca Cola (CFCC). To this end, we determined the genotoxicological and biological effects of different doses of lyophilised CCC and CFCC and Caffeine (CAF), the ma...

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Autores principales: Mateo Fernández, Marcos, Merinas Amo, Tania, Moreno Millán, Miguel, Alonso Moraga, Ángeles, Demyda Peyrás, Sebastián
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Biología
Biological and food safety
Coca Cola
Genotoxicological effects
Biological effects
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86057
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86057
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Biological and food safety
Coca Cola
Genotoxicological effects
Biological effects
spellingShingle Biología
Biological and food safety
Coca Cola
Genotoxicological effects
Biological effects
Mateo Fernández, Marcos
Merinas Amo, Tania
Moreno Millán, Miguel
Alonso Moraga, Ángeles
Demyda Peyrás, Sebastián
In Vivo and in Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
topic_facet Biología
Biological and food safety
Coca Cola
Genotoxicological effects
Biological effects
description The aim of this work was to assess the biological and food safety of two different beverages: Classic Coca Cola™ (CCC) and Caffeine-Free Coca Cola (CFCC). To this end, we determined the genotoxicological and biological effects of different doses of lyophilised CCC and CFCC and Caffeine (CAF), the main distinctive constituent. Their toxic/antitoxic, genotoxic/antigenotoxic, and chronic toxicity (lifespan assay) effects were determined in vivo using the Drosophila model. Their cytotoxic activities were determined using the HL-60 in vitro cancer model. In addition, clastogenic DNA toxicity was measured using internucleosomal fragmentation and SCGE assays. Their epigenetic effects were assessed on the HL-60 methylation status using some repetitive elements. The experimental results showed a slight chemopreventive effect of the two cola beverages against HL-60 leukaemia cells, probably mediated by nonapoptotic mechanisms. Finally, CCC and CAF induced a global genome hypomethylation evaluated in LINE-1 and Alu M1 repetitive elements. Overall, we demonstrated for the first time the safety of this famous beverage in in vivo and in vitro models.
format Articulo
Articulo
author Mateo Fernández, Marcos
Merinas Amo, Tania
Moreno Millán, Miguel
Alonso Moraga, Ángeles
Demyda Peyrás, Sebastián
author_facet Mateo Fernández, Marcos
Merinas Amo, Tania
Moreno Millán, Miguel
Alonso Moraga, Ángeles
Demyda Peyrás, Sebastián
author_sort Mateo Fernández, Marcos
title In Vivo and in Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title_short In Vivo and in Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title_full In Vivo and in Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title_fullStr In Vivo and in Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title_full_unstemmed In Vivo and in Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title_sort in vivo and in vitro genotoxic and epigenetic effects of two types of cola beverages and caffeine: a multiassay approach
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/86057
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