Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
Multiple-locus variable number-tandem repeat analysis (MLVA) of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Arg...
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| Autores principales: | , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2015
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/86053 |
| Aporte de: |
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I19-R120-10915-86053 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Veterinarias MIRU-VNTR MLVA Molecular typing Paratuberculosis |
| spellingShingle |
Ciencias Veterinarias MIRU-VNTR MLVA Molecular typing Paratuberculosis Gioffré, Andrea Correa Muñoz, Magnolia Alvarado Pinedo, María Fiorella Vaca, Roberto José Antonio Morsella, Claudia Fiorentino, María Andrea Paolicchi, Fernando Ruybal, Paula Zumárraga, Martín Travería, Gabriel Eduardo Romano, María Isabel Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis |
| topic_facet |
Ciencias Veterinarias MIRU-VNTR MLVA Molecular typing Paratuberculosis |
| description |
Multiple-locus variable number-tandem repeat analysis (MLVA) of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS<i>900</i>-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents. |
| format |
Articulo Articulo |
| author |
Gioffré, Andrea Correa Muñoz, Magnolia Alvarado Pinedo, María Fiorella Vaca, Roberto José Antonio Morsella, Claudia Fiorentino, María Andrea Paolicchi, Fernando Ruybal, Paula Zumárraga, Martín Travería, Gabriel Eduardo Romano, María Isabel |
| author_facet |
Gioffré, Andrea Correa Muñoz, Magnolia Alvarado Pinedo, María Fiorella Vaca, Roberto José Antonio Morsella, Claudia Fiorentino, María Andrea Paolicchi, Fernando Ruybal, Paula Zumárraga, Martín Travería, Gabriel Eduardo Romano, María Isabel |
| author_sort |
Gioffré, Andrea |
| title |
Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis |
| title_short |
Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis |
| title_full |
Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis |
| title_fullStr |
Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis |
| title_full_unstemmed |
Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis |
| title_sort |
molecular typing of argentinian <i>mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis |
| publishDate |
2015 |
| url |
http://sedici.unlp.edu.ar/handle/10915/86053 |
| work_keys_str_mv |
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Repositorios |
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1764820489249751043 |