A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis
Background: Bovine leukemia virus (BLV) is a member of retroviridae family, together with human T cell leukemia virus types 1 and 2 (HTLV-1 and -2) belonging to the genes deltaretrovirus, and infects cattle worldwide. Previous studies have classified the env sequences of BLV provirus from different...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2016
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/86046 |
| Aporte de: |
| id |
I19-R120-10915-86046 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Veterinarias BLV whole genome analysis Bovine leukemia virus (BLV) Gp51 env sequencing Next generation sequencing Phylogenetic analysis South America |
| spellingShingle |
Ciencias Veterinarias BLV whole genome analysis Bovine leukemia virus (BLV) Gp51 env sequencing Next generation sequencing Phylogenetic analysis South America Polat, Meripet Takeshima, Shin‑nosuke Hosomichi, Kazuyoshi Kim, Jiyun Kim Miyasaka, Taku Yamada, Kazunori Arainga, Mariluz Murakami, Tomoyuki Matsumoto, Yuki Barra Diaz, Veronica de la Panei, Carlos Javier González, Ester Teresa Kanemaki, Misao Onuma, Misao Giovambattista, Guillermo Aida, Yoko A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis |
| topic_facet |
Ciencias Veterinarias BLV whole genome analysis Bovine leukemia virus (BLV) Gp51 env sequencing Next generation sequencing Phylogenetic analysis South America |
| description |
Background: Bovine leukemia virus (BLV) is a member of retroviridae family, together with human T cell leukemia virus types 1 and 2 (HTLV-1 and -2) belonging to the genes deltaretrovirus, and infects cattle worldwide. Previous studies have classified the env sequences of BLV provirus from different geographic locations into eight genetic groups. To investigate the genetic variability of BLV in South America, we performed phylogenetic analyses of whole genome and partial env gp51 sequences of BLV strains isolated from Peru, Paraguay and Bolivia, for which no the molecular characteristics of BLV have previously been published, and discovered a novel BLV genotype, genotype-9, in Bolivia. Results: In Peru and Paraguay, 42.3 % (139/328) and over 50 % (76/139) of samples, respectively, were BLV positive. In Bolivia, the BLV infection rate was up to 30 % (156/507) at the individual level. In Argentina, 325/420 samples were BLV positive, with a BLV prevalence of 77.4 % at the individual level and up to 90.9 % at herd level. By contrast, relatively few BLV positive samples were detected in Chile, with a maximum of 29.1 % BLV infection at the individual level. We performed phylogenetic analyses using two different approaches, maximum likelihood (ML) tree and Bayesian inference, using 35 distinct partial env gp51 sequences from BLV strains isolated from Peru, Paraguay, and Bolivia, and 74 known BLV strains, representing eight different BLV genotypes from various geographical locations worldwide. The results indicated that Peruvian and Paraguayan BLV strains were grouped into genotypes-1, -2, and -6, while those from Bolivia were clustered into genotypes-1, -2, and -6, and a new genotype, genotype-9. Interestingly, these results were confirmed using ML phylogenetic analysis of whole genome sequences obtained by next generation sequencing of 25 BLV strains, assigned to four different genotypes (genotypes-1, -2, -6, and -9) from Peru, Paraguay, and Bolivia. Comparative analyses of complete genome sequences clearly showed some specific substitutions, in both structural and non-structural BLV genes, distinguishing the novel genotype-9 from known genotypes. Conclusions: Our results demonstrate widespread BLV infection in South American cattle and the existence of a new BLV genotype-9 in Bolivia. We conclude that at least seven BLV genotypes (genotypes-1, -2, -4, -5, -6, -7, and -9) are circulating in South America. |
| format |
Articulo Articulo |
| author |
Polat, Meripet Takeshima, Shin‑nosuke Hosomichi, Kazuyoshi Kim, Jiyun Kim Miyasaka, Taku Yamada, Kazunori Arainga, Mariluz Murakami, Tomoyuki Matsumoto, Yuki Barra Diaz, Veronica de la Panei, Carlos Javier González, Ester Teresa Kanemaki, Misao Onuma, Misao Giovambattista, Guillermo Aida, Yoko |
| author_facet |
Polat, Meripet Takeshima, Shin‑nosuke Hosomichi, Kazuyoshi Kim, Jiyun Kim Miyasaka, Taku Yamada, Kazunori Arainga, Mariluz Murakami, Tomoyuki Matsumoto, Yuki Barra Diaz, Veronica de la Panei, Carlos Javier González, Ester Teresa Kanemaki, Misao Onuma, Misao Giovambattista, Guillermo Aida, Yoko |
| author_sort |
Polat, Meripet |
| title |
A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis |
| title_short |
A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis |
| title_full |
A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis |
| title_fullStr |
A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis |
| title_full_unstemmed |
A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis |
| title_sort |
new genotype of bovine leukemia virus in south america identified by ngs-based whole genome sequencing and molecular evolutionary genetic analysis |
| publishDate |
2016 |
| url |
http://sedici.unlp.edu.ar/handle/10915/86046 |
| work_keys_str_mv |
AT polatmeripet anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT takeshimashinnosuke anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT hosomichikazuyoshi anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT kimjiyunkim anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT miyasakataku anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT yamadakazunori anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT araingamariluz anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT murakamitomoyuki anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT matsumotoyuki anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT barradiazveronicadela anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT paneicarlosjavier anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT gonzalezesterteresa anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT kanemakimisao anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT onumamisao anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT giovambattistaguillermo anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT aidayoko anewgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT polatmeripet newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT takeshimashinnosuke newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT hosomichikazuyoshi newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT kimjiyunkim newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT miyasakataku newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT yamadakazunori newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT araingamariluz newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT murakamitomoyuki newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT matsumotoyuki newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT barradiazveronicadela newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT paneicarlosjavier newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT gonzalezesterteresa newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT kanemakimisao newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT onumamisao newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT giovambattistaguillermo newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis AT aidayoko newgenotypeofbovineleukemiavirusinsouthamericaidentifiedbyngsbasedwholegenomesequencingandmolecularevolutionarygeneticanalysis |
| bdutipo_str |
Repositorios |
| _version_ |
1764820489242411008 |