In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots

Azospirillum brasilense is a soil bacterium capable of promoting plant growth. Several surface components were previously reported to be involved in the attachment of A. brasilense to root plants. Among these components are the exopolysaccharide (EPS), lipopolysaccharide (LPS) and the polar flagellu...

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Autores principales: Rossi, Fernando Ariel, Medeot, Daniela Beatriz, Liaudat, Juan Pablo, Pistorio, Mariano, Jofré, Edgardo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Biología
Exopolysaccharides
Lipopolysaccharides
Motility
Root-adsorption
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85991
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85991
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Exopolysaccharides
Lipopolysaccharides
Motility
Root-adsorption
spellingShingle Biología
Exopolysaccharides
Lipopolysaccharides
Motility
Root-adsorption
Rossi, Fernando Ariel
Medeot, Daniela Beatriz
Liaudat, Juan Pablo
Pistorio, Mariano
Jofré, Edgardo
In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
topic_facet Biología
Exopolysaccharides
Lipopolysaccharides
Motility
Root-adsorption
description Azospirillum brasilense is a soil bacterium capable of promoting plant growth. Several surface components were previously reported to be involved in the attachment of A. brasilense to root plants. Among these components are the exopolysaccharide (EPS), lipopolysaccharide (LPS) and the polar flagellum. Flagellin from polar flagellum is glycosylated and it was suggested that genes involved in such a posttranslational modification are the same ones involved in the biosynthesis of sugars present in the O-antigen of the LPS. In this work, we report on the characterization of two homologs present in A. brasilense Cd, to the well characterized flagellin modification genes, flmA and flmB, from Aeromonas caviae. We show that mutations in either flmA or flmB genes of A. brasilense resulted in non-motile cells due to alterations in the polar flagellum assembly. Moreover, these mutations also affected the capability of A. brasilense cells to adsorb to maize roots and to produce LPS and EPS. By generating a mutant containing the polar flagellum affected in their rotation, we show the importance of the bacterial motility for the early colonization of maize roots.
format Articulo
Articulo
author Rossi, Fernando Ariel
Medeot, Daniela Beatriz
Liaudat, Juan Pablo
Pistorio, Mariano
Jofré, Edgardo
author_facet Rossi, Fernando Ariel
Medeot, Daniela Beatriz
Liaudat, Juan Pablo
Pistorio, Mariano
Jofré, Edgardo
author_sort Rossi, Fernando Ariel
title In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
title_short In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
title_full In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
title_fullStr In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
title_full_unstemmed In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
title_sort in azospirillum brasilense, mutations in flma or flmb genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/85991
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bdutipo_str Repositorios
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