The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30)

The sodium/bicarbonate cotransporter (NBC) transports extracellular Na+ and HCO3 − into the cytoplasm upon intracellular acidosis, restoring the acidic pHi to near neutral values. Two different NBC isoforms have been described in the heart, the electroneutral NBCn1 (1Na+:1HCO3 −) and the electrogeni...

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Detalles Bibliográficos
Autores principales: Orlowski, Alejandro, De Giusti, Verónica Celeste, Ciancio, María Carolina, Espejo, María Sofía, Aiello, Ernesto Alejandro
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Médicas
aldosterone
electrogenic
GPR30
heart
sodium/bicarbonate cotransporter
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85976
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85976
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
aldosterone
electrogenic
GPR30
heart
sodium/bicarbonate cotransporter
spellingShingle Ciencias Médicas
aldosterone
electrogenic
GPR30
heart
sodium/bicarbonate cotransporter
Orlowski, Alejandro
De Giusti, Verónica Celeste
Ciancio, María Carolina
Espejo, María Sofía
Aiello, Ernesto Alejandro
The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30)
topic_facet Ciencias Médicas
aldosterone
electrogenic
GPR30
heart
sodium/bicarbonate cotransporter
description The sodium/bicarbonate cotransporter (NBC) transports extracellular Na+ and HCO3 − into the cytoplasm upon intracellular acidosis, restoring the acidic pHi to near neutral values. Two different NBC isoforms have been described in the heart, the electroneutral NBCn1 (1Na+:1HCO3 −) and the electrogenic NBCe1 (1Na+:2HCO3 −). Certain non-genomic effects of aldosterone (Ald) were due to an orphan G protein-couple receptor 30 (GPR30). We have recently demonstrated that Ald activates GPR30 in adult rat ventricular myocytes, which transactivates the epidermal growth factor receptor (EGFR) and in turn triggers a reactive oxygen species (ROS)- and PI3K/AKT-dependent pathway, leading to the stimulation of NBC. The aim of this study was to investigate the NBC isoform involved in the Ald/GPR30-induced NBC activation. Using specific NBCe1 inhibitory antibodies (a-L3) we demonstrated that Ald does not affect NBCn1 activity. Ald was able to increase NBCe1 activity recorded in isolation. Using immunofluorescence and confocal microscopy analysis we showed in this work that both NBCe1 and GPR30 are localized in t-tubules. In conclusion, we have demonstrated that NBCe1 is the NBC isoform activated by Ald in the heart.
format Articulo
Articulo
author Orlowski, Alejandro
De Giusti, Verónica Celeste
Ciancio, María Carolina
Espejo, María Sofía
Aiello, Ernesto Alejandro
author_facet Orlowski, Alejandro
De Giusti, Verónica Celeste
Ciancio, María Carolina
Espejo, María Sofía
Aiello, Ernesto Alejandro
author_sort Orlowski, Alejandro
title The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30)
title_short The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30)
title_full The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30)
title_fullStr The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30)
title_full_unstemmed The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30)
title_sort cardiac electrogenic sodium/bicarbonate cotransporter (nbce1) is activated by aldosterone through the g protein-coupled receptor 30 (gpr 30)
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/85976
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