Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner
Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fash...
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Autores principales: | , , , , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
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2016
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/85969 |
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I19-R120-10915-85969 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
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SEDICI (UNLP) |
language |
Inglés |
topic |
Ciencias Médicas Lactate GPR81 Macrophage pro-inflammatory |
spellingShingle |
Ciencias Médicas Lactate GPR81 Macrophage pro-inflammatory Errea, Agustina Juliana Cayet, Delphine Marchetti, Philippe Tang, Cong Kluza, Jerome Offermanns, Stefan Sirard, Jean-Claude Rumbo, Martín Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner |
topic_facet |
Ciencias Médicas Lactate GPR81 Macrophage pro-inflammatory |
description |
Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/-cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/-cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation. |
format |
Articulo Articulo |
author |
Errea, Agustina Juliana Cayet, Delphine Marchetti, Philippe Tang, Cong Kluza, Jerome Offermanns, Stefan Sirard, Jean-Claude Rumbo, Martín |
author_facet |
Errea, Agustina Juliana Cayet, Delphine Marchetti, Philippe Tang, Cong Kluza, Jerome Offermanns, Stefan Sirard, Jean-Claude Rumbo, Martín |
author_sort |
Errea, Agustina Juliana |
title |
Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner |
title_short |
Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner |
title_full |
Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner |
title_fullStr |
Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner |
title_full_unstemmed |
Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner |
title_sort |
lactate inhibits the pro-inflammatory response and metabolic reprogramming in murine macrophages in a gpr81-independent manner |
publishDate |
2016 |
url |
http://sedici.unlp.edu.ar/handle/10915/85969 |
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Repositorios |
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