A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement

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Introduction: Mesenchymal stem cells (MSCs) are a promising source of cells for regenerative therapies. Although they can be isolated easily from several tissues, cell expansion is limited since their properties are lost with successive passages. Hence, pluripotent derived MSCs (PD-MSCs) arise as a...

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Autores principales: Luzzani, Carlos, Neiman, Gabriel, Garate, Ximena, Questa, María, Solari, Claudia, Fernández Espinosa, Darío, García, Marcela Nilda, Errecalde, Ana Lía, Guberman, Alejandra, Scassa, María Élida, Sevlever, Gustavo Emilio, Romorini, Leonardo, Miriuka, Santiago Gabriel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Médicas
Embryonic Stem Cells
Cell Line
HESC lines
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85961
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85961
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Embryonic Stem Cells
Cell Line
HESC lines
spellingShingle Ciencias Médicas
Embryonic Stem Cells
Cell Line
HESC lines
Luzzani, Carlos
Neiman, Gabriel
Garate, Ximena
Questa, María
Solari, Claudia
Fernández Espinosa, Darío
García, Marcela Nilda
Errecalde, Ana Lía
Guberman, Alejandra
Scassa, María Élida
Sevlever, Gustavo Emilio
Romorini, Leonardo
Miriuka, Santiago Gabriel
A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
topic_facet Ciencias Médicas
Embryonic Stem Cells
Cell Line
HESC lines
description Introduction: Mesenchymal stem cells (MSCs) are a promising source of cells for regenerative therapies. Although they can be isolated easily from several tissues, cell expansion is limited since their properties are lost with successive passages. Hence, pluripotent derived MSCs (PD-MSCs) arise as a suitable alternative for MSC production. Nevertheless, at present, PD-MSC derivation protocols are either expensive or not suitable for clinical purposes. Methods: In this work we present a therapy-grade, inexpensive and simple protocol to derive MSCs from pluripotent stem cells (PSCs) based on the use of platelet lysate (PL) as medium supplement. Results: We showed that the PD-MSC<SUB>PL</SUB> expressed multiple MSC markers, including CD90, CD73, CD105, CD166, and CD271, among others. These cells also show multilineage differentiation ability and immunomodulatory effects on pre-stimulated lymphocytes. Thorough characterization of these cells showed that a PD-MSC<SUB>PL</SUB> resembles an umbilical cord (UC) MSC and differs from a PSC in surface marker and extracellular matrix proteins and integrin expression. Moreover, the OCT-4 promoter is re-methylated with mesenchymal differentiation comparable with the methylation levels of UC-MSCs and fibroblasts. Lastly, the use of PL-supplemented medium generates significantly more MSCs than the use of fetal bovine serum. Conclusions: This protocol can be used to generate a large amount of PD-MSCs with low cost and is compatible with clinical therapies.
format Articulo
Articulo
author Luzzani, Carlos
Neiman, Gabriel
Garate, Ximena
Questa, María
Solari, Claudia
Fernández Espinosa, Darío
García, Marcela Nilda
Errecalde, Ana Lía
Guberman, Alejandra
Scassa, María Élida
Sevlever, Gustavo Emilio
Romorini, Leonardo
Miriuka, Santiago Gabriel
author_facet Luzzani, Carlos
Neiman, Gabriel
Garate, Ximena
Questa, María
Solari, Claudia
Fernández Espinosa, Darío
García, Marcela Nilda
Errecalde, Ana Lía
Guberman, Alejandra
Scassa, María Élida
Sevlever, Gustavo Emilio
Romorini, Leonardo
Miriuka, Santiago Gabriel
author_sort Luzzani, Carlos
title A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
title_short A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
title_full A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
title_fullStr A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
title_full_unstemmed A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
title_sort therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/85961
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