Muc5ac mucin expression during rat skin development

Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during pre-and post-natal rat...

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Autores principales: Ferretti, Valeria Alejandra, Segal-Eiras, Amada, Barbeito, Claudio Gustavo, Croce, María Virginia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Biología
Development
Muc5ac
Rat
Skin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85831
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85831
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Development
Muc5ac
Rat
Skin
spellingShingle Biología
Development
Muc5ac
Rat
Skin
Ferretti, Valeria Alejandra
Segal-Eiras, Amada
Barbeito, Claudio Gustavo
Croce, María Virginia
Muc5ac mucin expression during rat skin development
topic_facet Biología
Development
Muc5ac
Rat
Skin
description Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during pre-and post-natal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report that confirmed Muc5ac expression during skin development. and airways epithelia.2 It is common knowledge that normal skin exhibits a rather restricted expression of mucins, and it has been demonstrated that epithelial cells of the normal epidermis do not express sialomucins while Muc5ac expression in cancer epidermal cells is well documented.4,5 On the other hand, little is known about mucin expression in epidermal development, neither in humans nor in other species. However, some mucin genes have been detected during human embryonic and fetal organ development according to differential expression patterns in comparison with adult tissues.3,6 The present research was developed to explore Muc5ac skin expression during preand post-natal rat development.
format Articulo
Articulo
author Ferretti, Valeria Alejandra
Segal-Eiras, Amada
Barbeito, Claudio Gustavo
Croce, María Virginia
author_facet Ferretti, Valeria Alejandra
Segal-Eiras, Amada
Barbeito, Claudio Gustavo
Croce, María Virginia
author_sort Ferretti, Valeria Alejandra
title Muc5ac mucin expression during rat skin development
title_short Muc5ac mucin expression during rat skin development
title_full Muc5ac mucin expression during rat skin development
title_fullStr Muc5ac mucin expression during rat skin development
title_full_unstemmed Muc5ac mucin expression during rat skin development
title_sort muc5ac mucin expression during rat skin development
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/85831
work_keys_str_mv AT ferrettivaleriaalejandra muc5acmucinexpressionduringratskindevelopment
AT segaleirasamada muc5acmucinexpressionduringratskindevelopment
AT barbeitoclaudiogustavo muc5acmucinexpressionduringratskindevelopment
AT crocemariavirginia muc5acmucinexpressionduringratskindevelopment
bdutipo_str Repositorios
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