Aging and CaMKII alter intracellular Ca2+ transients and heart rhythm in Drosophila melanogaster

Aging is associated to disrupted contractility and rhythmicity, among other cardiovascular alterations. Drosophila melanogaster shows a pattern of aging similar to human beings and recapitulates the arrhythmogenic conditions found in the human heart. Moreover, the kinase CaMKII has been characterize...

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Autores principales: Santalla, Manuela, Valverde, Carlos Alfredo, Harnichar, E., Lacunza, Ezequiel, Aguilar Fuentes, J., Mattiazzi, Alicia Ramona, Ferrero, Paola Viviana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Médicas
Drosophila melanogaster
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85638
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85638
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Drosophila melanogaster
spellingShingle Ciencias Médicas
Drosophila melanogaster
Santalla, Manuela
Valverde, Carlos Alfredo
Harnichar, E.
Lacunza, Ezequiel
Aguilar Fuentes, J.
Mattiazzi, Alicia Ramona
Ferrero, Paola Viviana
Aging and CaMKII alter intracellular Ca2+ transients and heart rhythm in Drosophila melanogaster
topic_facet Ciencias Médicas
Drosophila melanogaster
description Aging is associated to disrupted contractility and rhythmicity, among other cardiovascular alterations. Drosophila melanogaster shows a pattern of aging similar to human beings and recapitulates the arrhythmogenic conditions found in the human heart. Moreover, the kinase CaMKII has been characterized as an important regulator of heart function and an arrhythmogenic molecule that participate in Ca2+ handling. Using a genetically engineered expressed Ca2+ indicator, we report changes in cardiac Ca 2+ handling at two different ages. Aging prolonged relaxation, reduced spontaneous heart rate (HR) and increased the occurrence of arrhythmias, ectopic beats and asystoles. Alignment between Drosophila melanogaster and human CaMKII showed a high degree of conservation and indicates that relevant phosphorylation sites in humans are also present in the fruit fly. Inhibition of CaMKII by KN-93 (CaMKII-specific inhibitor), reduced HR without significant changes in other parameters. By contrast, overexpression of CaMKII increased HR and reduced arrhythmias. Moreover, it increased fluorescence amplitude, maximal rate of rise of fluorescence and reduced time to peak fluorescence. These results suggest that CaMKII in Drosophila melanogaster acts directly on heart function and that increasing CaMKII expression levels could be beneficial to improve contractility.
format Articulo
Articulo
author Santalla, Manuela
Valverde, Carlos Alfredo
Harnichar, E.
Lacunza, Ezequiel
Aguilar Fuentes, J.
Mattiazzi, Alicia Ramona
Ferrero, Paola Viviana
author_facet Santalla, Manuela
Valverde, Carlos Alfredo
Harnichar, E.
Lacunza, Ezequiel
Aguilar Fuentes, J.
Mattiazzi, Alicia Ramona
Ferrero, Paola Viviana
author_sort Santalla, Manuela
title Aging and CaMKII alter intracellular Ca2+ transients and heart rhythm in Drosophila melanogaster
title_short Aging and CaMKII alter intracellular Ca2+ transients and heart rhythm in Drosophila melanogaster
title_full Aging and CaMKII alter intracellular Ca2+ transients and heart rhythm in Drosophila melanogaster
title_fullStr Aging and CaMKII alter intracellular Ca2+ transients and heart rhythm in Drosophila melanogaster
title_full_unstemmed Aging and CaMKII alter intracellular Ca2+ transients and heart rhythm in Drosophila melanogaster
title_sort aging and camkii alter intracellular ca2+ transients and heart rhythm in drosophila melanogaster
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85638
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