Genetically encoded sender-receiver system in 3D mammalian cell culture

Engineering spatial patterning in mammalian cells, employing entirely genetically encoded components, requires solving several problems. These include how to code secreted activator or inhibitor molecules and how to send concentration-dependent signals to neighboring cells, to control gene expressio...

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Detalles Bibliográficos
Autores principales: Carvalho, A., Bárcena Menéndez, Diego, Senthivel, V. R., Zimmermann, T., Diambra, Luis Aníbal, Isalan, M.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Exactas
HGF
MDCK
morphogen
NK4
synthetic patterning
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85585
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85585
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
HGF
MDCK
morphogen
NK4
synthetic patterning
spellingShingle Ciencias Exactas
HGF
MDCK
morphogen
NK4
synthetic patterning
Carvalho, A.
Bárcena Menéndez, Diego
Senthivel, V. R.
Zimmermann, T.
Diambra, Luis Aníbal
Isalan, M.
Genetically encoded sender-receiver system in 3D mammalian cell culture
topic_facet Ciencias Exactas
HGF
MDCK
morphogen
NK4
synthetic patterning
description Engineering spatial patterning in mammalian cells, employing entirely genetically encoded components, requires solving several problems. These include how to code secreted activator or inhibitor molecules and how to send concentration-dependent signals to neighboring cells, to control gene expression. The Madin-Darby Canine Kidney (MDCK) cell line is a potential engineering scaffold as it forms hollow spheres (cysts) in 3D culture and tubulates in response to extracellular hepatocyte growth factor (HGF). We first aimed to graft a synthetic patterning system onto single developing MDCK cysts. We therefore developed a new localized transfection method to engineer distinct sender and receiver regions. A stable reporter line enabled reversible EGFP activation by HGF and modulation by a secreted repressor (a truncated HGF variant, NK4). By expanding the scale to wide fields of cysts, we generated morphogen diffusion gradients, controlling reporter gene expression. Together, these components provide a toolkit for engineering cell-cell communication networks in 3D cell culture.
format Articulo
Articulo
author Carvalho, A.
Bárcena Menéndez, Diego
Senthivel, V. R.
Zimmermann, T.
Diambra, Luis Aníbal
Isalan, M.
author_facet Carvalho, A.
Bárcena Menéndez, Diego
Senthivel, V. R.
Zimmermann, T.
Diambra, Luis Aníbal
Isalan, M.
author_sort Carvalho, A.
title Genetically encoded sender-receiver system in 3D mammalian cell culture
title_short Genetically encoded sender-receiver system in 3D mammalian cell culture
title_full Genetically encoded sender-receiver system in 3D mammalian cell culture
title_fullStr Genetically encoded sender-receiver system in 3D mammalian cell culture
title_full_unstemmed Genetically encoded sender-receiver system in 3D mammalian cell culture
title_sort genetically encoded sender-receiver system in 3d mammalian cell culture
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85585
work_keys_str_mv AT carvalhoa geneticallyencodedsenderreceiversystemin3dmammaliancellculture
AT barcenamenendezdiego geneticallyencodedsenderreceiversystemin3dmammaliancellculture
AT senthivelvr geneticallyencodedsenderreceiversystemin3dmammaliancellculture
AT zimmermannt geneticallyencodedsenderreceiversystemin3dmammaliancellculture
AT diambraluisanibal geneticallyencodedsenderreceiversystemin3dmammaliancellculture
AT isalanm geneticallyencodedsenderreceiversystemin3dmammaliancellculture
bdutipo_str Repositorios
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