Increased Na+/Ca2+ exchanger expression/activity constitutes a point of inflection in the progression to heart failure of hypertensive rats

Spontaneously hypertensive rat (SHR) constitutes a genetic model widely used to study the natural evolution of hypertensive heart disease. Ca 2+-handling alterations are known to occur in SHR. However, the putative modifications of Ca2+-handling proteins during the progression to heart failure (HF)...

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Autores principales: Rodriguez, Jesica S., Vélez Rueda, Jorge Omar, Salas, Margarita Ana, Becerra, Romina Valeria, Di Carlo, Mariano Nahuel, Said, María Matilde, Vittone, Leticia, Rinaldi, Gustavo, Portiansky, Enrique Leo, Mundiña-Weilenmann, Cecilia, Palomeque, Julieta, Mattiazzi, Alicia Ramona
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Médicas
Intercambiador de Sodio-Calcio
Hipertensión
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85568
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85568
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Intercambiador de Sodio-Calcio
Hipertensión
spellingShingle Ciencias Médicas
Intercambiador de Sodio-Calcio
Hipertensión
Rodriguez, Jesica S.
Vélez Rueda, Jorge Omar
Salas, Margarita Ana
Becerra, Romina Valeria
Di Carlo, Mariano Nahuel
Said, María Matilde
Vittone, Leticia
Rinaldi, Gustavo
Portiansky, Enrique Leo
Mundiña-Weilenmann, Cecilia
Palomeque, Julieta
Mattiazzi, Alicia Ramona
Increased Na+/Ca2+ exchanger expression/activity constitutes a point of inflection in the progression to heart failure of hypertensive rats
topic_facet Ciencias Médicas
Intercambiador de Sodio-Calcio
Hipertensión
description Spontaneously hypertensive rat (SHR) constitutes a genetic model widely used to study the natural evolution of hypertensive heart disease. Ca 2+-handling alterations are known to occur in SHR. However, the putative modifications of Ca2+-handling proteins during the progression to heart failure (HF) are not well established. Moreover, the role of apoptosis in SHR is controversial. We investigated intracellular Ca 2+, Ca2+-handling proteins and apoptosis in SHR vs. control Wistar rats (W) from 3 to 15 months (mo). Changes associated with the transition to HF (i.e. lung edema and decrease in midwall fractional shortening), occurred at 15 mo in 38% of SHR (SHRF). In SHRF, twitch and caffeine-induced Ca2+ transients, significantly decreased relative to 6/9 mo and 15 mo without HF signs. This decrease occurred in association with a decrease in the time constant of caffeine-Ca2+ transient decay and an increase in Na+/Ca2+ exchanger (NCX) abundance (p< 0.05) with no changes in SERCA2a expression/activity. An increased Ca 2+-calmodulin-kinase II activity, associated with an enhancement of apoptosis (TUNEL and Bax/Bcl2) was observed in SHR relative to W from 3 to 15 mo. Conclusions: 1. Apoptosis is an early and persistent event that may contribute to hypertrophic remodeling but would not participate in the contractile impairment of SHRF. 2. The increase in NCX expression/activity, associated with an increase in Ca2+ efflux from the cell, constitutes a primary alteration of Ca2+-handling proteins in the evolution to HF. 3. No changes in SERCA2a expression/activity are observed when HF signs become evident.
format Articulo
Articulo
author Rodriguez, Jesica S.
Vélez Rueda, Jorge Omar
Salas, Margarita Ana
Becerra, Romina Valeria
Di Carlo, Mariano Nahuel
Said, María Matilde
Vittone, Leticia
Rinaldi, Gustavo
Portiansky, Enrique Leo
Mundiña-Weilenmann, Cecilia
Palomeque, Julieta
Mattiazzi, Alicia Ramona
author_facet Rodriguez, Jesica S.
Vélez Rueda, Jorge Omar
Salas, Margarita Ana
Becerra, Romina Valeria
Di Carlo, Mariano Nahuel
Said, María Matilde
Vittone, Leticia
Rinaldi, Gustavo
Portiansky, Enrique Leo
Mundiña-Weilenmann, Cecilia
Palomeque, Julieta
Mattiazzi, Alicia Ramona
author_sort Rodriguez, Jesica S.
title Increased Na+/Ca2+ exchanger expression/activity constitutes a point of inflection in the progression to heart failure of hypertensive rats
title_short Increased Na+/Ca2+ exchanger expression/activity constitutes a point of inflection in the progression to heart failure of hypertensive rats
title_full Increased Na+/Ca2+ exchanger expression/activity constitutes a point of inflection in the progression to heart failure of hypertensive rats
title_fullStr Increased Na+/Ca2+ exchanger expression/activity constitutes a point of inflection in the progression to heart failure of hypertensive rats
title_full_unstemmed Increased Na+/Ca2+ exchanger expression/activity constitutes a point of inflection in the progression to heart failure of hypertensive rats
title_sort increased na+/ca2+ exchanger expression/activity constitutes a point of inflection in the progression to heart failure of hypertensive rats
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85568
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