Métodos electroforético e inmunoquímicos para detectar proteínas de leche, soja y huevo en premezclas comerciales

The presence of milk, egg and soy proteins was evaluated in thirteen commercial products, which were premixes to make cream caramel, cake, soy “milanesas”, “tortas fritas”, pizza, gnocchi and complementary foods. Samples were analysed using SDSPAGE, immunoblotting and ELISA methods. The electrophore...

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Autores principales: Cagnasso, Carolina Elisa, Martín, María Eugenia, Cellerino, Karina, Audisio, Fabiola, Docena, Guillermo Horacio, Polenta, Gustavo, López, Laura Beatriz
Formato: Articulo
Lenguaje:Español
Publicado: 2014
Materias:
Ciencias Exactas
Egg
Food allergens
Immunochemical methods
Milk
Soy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85505
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85505
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Exactas
Egg
Food allergens
Immunochemical methods
Milk
Soy
spellingShingle Ciencias Exactas
Egg
Food allergens
Immunochemical methods
Milk
Soy
Cagnasso, Carolina Elisa
Martín, María Eugenia
Cellerino, Karina
Audisio, Fabiola
Docena, Guillermo Horacio
Polenta, Gustavo
López, Laura Beatriz
Métodos electroforético e inmunoquímicos para detectar proteínas de leche, soja y huevo en premezclas comerciales
topic_facet Ciencias Exactas
Egg
Food allergens
Immunochemical methods
Milk
Soy
description The presence of milk, egg and soy proteins was evaluated in thirteen commercial products, which were premixes to make cream caramel, cake, soy “milanesas”, “tortas fritas”, pizza, gnocchi and complementary foods. Samples were analysed using SDSPAGE, immunoblotting and ELISA methods. The electrophoretic method and immunoblotting were useful to confirm the presence of milk, egg and soy proteins in four samples that declared them as ingredients. In other samples, both methods showed negative results for some of these proteins, although they were also declared as ingredients. This suggests that those proteins were not added as ingredients in these products. The ELISA kits detected very low concentration of the allergenic proteins in four products with precautionary phrases and also in five samples that made no reference to them in their labels. ELISA methods are useful to detect cross contamination due to their high sensitivity. The food industry should be responsible for the declaration of milk, egg and soy proteins in their food labels.
format Articulo
Articulo
author Cagnasso, Carolina Elisa
Martín, María Eugenia
Cellerino, Karina
Audisio, Fabiola
Docena, Guillermo Horacio
Polenta, Gustavo
López, Laura Beatriz
author_facet Cagnasso, Carolina Elisa
Martín, María Eugenia
Cellerino, Karina
Audisio, Fabiola
Docena, Guillermo Horacio
Polenta, Gustavo
López, Laura Beatriz
author_sort Cagnasso, Carolina Elisa
title Métodos electroforético e inmunoquímicos para detectar proteínas de leche, soja y huevo en premezclas comerciales
title_short Métodos electroforético e inmunoquímicos para detectar proteínas de leche, soja y huevo en premezclas comerciales
title_full Métodos electroforético e inmunoquímicos para detectar proteínas de leche, soja y huevo en premezclas comerciales
title_fullStr Métodos electroforético e inmunoquímicos para detectar proteínas de leche, soja y huevo en premezclas comerciales
title_full_unstemmed Métodos electroforético e inmunoquímicos para detectar proteínas de leche, soja y huevo en premezclas comerciales
title_sort métodos electroforético e inmunoquímicos para detectar proteínas de leche, soja y huevo en premezclas comerciales
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85505
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