Effects of Different Anesthetics in the Murine Model of EHV-1 Infection

Mice are commonly used as an experimental model to investigate the Equid herpesvirus 1 (EHV-1) infection. This model easily reproduces the disease, and the clinical signs are more or less similar to those observed in the horse, the natural host. During natural infection, the acute course of respirat...

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Autores principales: Eöry, Matías Leonel, Zanuzzi, Carolina Natalia, Fuentealba, Nadia Analía, Sguazza, Guillermo Hernán, Gimeno, Eduardo Juan, Galosi, Cecilia Mónica, Barbeito, Claudio Gustavo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Veterinarias
anesthetics
apoptosis
Equid Herpesvirus 1
immunohistochemistry
mice
proliferation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85493
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85493
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
anesthetics
apoptosis
Equid Herpesvirus 1
immunohistochemistry
mice
proliferation
spellingShingle Ciencias Veterinarias
anesthetics
apoptosis
Equid Herpesvirus 1
immunohistochemistry
mice
proliferation
Eöry, Matías Leonel
Zanuzzi, Carolina Natalia
Fuentealba, Nadia Analía
Sguazza, Guillermo Hernán
Gimeno, Eduardo Juan
Galosi, Cecilia Mónica
Barbeito, Claudio Gustavo
Effects of Different Anesthetics in the Murine Model of EHV-1 Infection
topic_facet Ciencias Veterinarias
anesthetics
apoptosis
Equid Herpesvirus 1
immunohistochemistry
mice
proliferation
description Mice are commonly used as an experimental model to investigate the Equid herpesvirus 1 (EHV-1) infection. This model easily reproduces the disease, and the clinical signs are more or less similar to those observed in the horse, the natural host. During natural infection, the acute course of respiratory infection is mandatory for the development of adaptive immune response. Since interactions between EHV-1 and anesthetics are possible, the study investigated whether the early events of murine pulmonary immune response could be affected by different anesthetics. Therefore, mice were experimentally infected with a unique EHV-1 strain under the effects of ether, ketamine/xylazine, or isoflurane. Clinical signs and histopathological lesions in the lungs were described, and the cell death and proliferation rates of sham-inoculated or infected animals were quantified using immunohistochemistry. Clinical signs were more severe in animals anesthetized with ether. Qualitative differences in the recruited inflammatory cells were observed following application of anesthesia. The level of infection between the infected groups was not statistically significant. However, lungs from ketamine/xylazine-anesthetized animals showed the highest cell death rates, whereas those from isoflurane-anesthetized animals showed the highest proliferation rates. It has been emphasized that anesthetics alone or their interactions with EHV-1 modify the response against the infection. An appropriate selection of the anesthetic during experimental studies is relevant to minimize wrong conclusions.
format Articulo
Articulo
author Eöry, Matías Leonel
Zanuzzi, Carolina Natalia
Fuentealba, Nadia Analía
Sguazza, Guillermo Hernán
Gimeno, Eduardo Juan
Galosi, Cecilia Mónica
Barbeito, Claudio Gustavo
author_facet Eöry, Matías Leonel
Zanuzzi, Carolina Natalia
Fuentealba, Nadia Analía
Sguazza, Guillermo Hernán
Gimeno, Eduardo Juan
Galosi, Cecilia Mónica
Barbeito, Claudio Gustavo
author_sort Eöry, Matías Leonel
title Effects of Different Anesthetics in the Murine Model of EHV-1 Infection
title_short Effects of Different Anesthetics in the Murine Model of EHV-1 Infection
title_full Effects of Different Anesthetics in the Murine Model of EHV-1 Infection
title_fullStr Effects of Different Anesthetics in the Murine Model of EHV-1 Infection
title_full_unstemmed Effects of Different Anesthetics in the Murine Model of EHV-1 Infection
title_sort effects of different anesthetics in the murine model of ehv-1 infection
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/85493
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