Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease

The MICA/B genes (<i>MHC class I chain related genes A and B</i>) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral...

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Autores principales: Allegretti, Yessica Lorena, Bondar, Constanza María, Guzman, Luciana, Cueto Rua, Eduardo, Chopita, Néstor Alfredo, Fuertes, Mercedes, Zwirner, Norberto W., Chirdo, Fernando Gabriel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Exactas
Ciencias Médicas
Celiac Disease
Cellular Stress
MICA/B genes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85478
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85478
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Ciencias Médicas
Celiac Disease
Cellular Stress
MICA/B genes
spellingShingle Ciencias Exactas
Ciencias Médicas
Celiac Disease
Cellular Stress
MICA/B genes
Allegretti, Yessica Lorena
Bondar, Constanza María
Guzman, Luciana
Cueto Rua, Eduardo
Chopita, Néstor Alfredo
Fuertes, Mercedes
Zwirner, Norberto W.
Chirdo, Fernando Gabriel
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
topic_facet Ciencias Exactas
Ciencias Médicas
Celiac Disease
Cellular Stress
MICA/B genes
description The MICA/B genes (<i>MHC class I chain related genes A and B</i>) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B<SUP>+</SUP> T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B<SUP>+</SUP> B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in <i>in vitro</i> models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role.
format Articulo
Articulo
author Allegretti, Yessica Lorena
Bondar, Constanza María
Guzman, Luciana
Cueto Rua, Eduardo
Chopita, Néstor Alfredo
Fuertes, Mercedes
Zwirner, Norberto W.
Chirdo, Fernando Gabriel
author_facet Allegretti, Yessica Lorena
Bondar, Constanza María
Guzman, Luciana
Cueto Rua, Eduardo
Chopita, Néstor Alfredo
Fuertes, Mercedes
Zwirner, Norberto W.
Chirdo, Fernando Gabriel
author_sort Allegretti, Yessica Lorena
title Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title_short Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title_full Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title_fullStr Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title_full_unstemmed Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title_sort broad mica/b expression in the small bowel mucosa: a link between cellular stress and celiac disease
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/85478
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