Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, w...

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Detalles Bibliográficos
Autores principales: Querido, Jailson F. B., Agirre, Jon, Martí, Gerardo Aníbal, Guérin, Diego M. A., Silva, Marcelo Sousa
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Biología
Triatoma virus
Triatomines
Identification method
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85477
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.

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