Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, w...

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Autores principales: Querido, Jailson F. B., Agirre, Jon, Martí, Gerardo Aníbal, Guérin, Diego M. A., Silva, Marcelo Sousa
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Biología
Triatoma virus
Triatomines
Identification method
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85477
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85477
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Triatoma virus
Triatomines
Identification method
spellingShingle Biología
Triatoma virus
Triatomines
Identification method
Querido, Jailson F. B.
Agirre, Jon
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Silva, Marcelo Sousa
Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
topic_facet Biología
Triatoma virus
Triatomines
Identification method
description Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
format Articulo
Articulo
author Querido, Jailson F. B.
Agirre, Jon
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Silva, Marcelo Sousa
author_facet Querido, Jailson F. B.
Agirre, Jon
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Silva, Marcelo Sousa
author_sort Querido, Jailson F. B.
title Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_short Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_full Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_fullStr Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_full_unstemmed Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_sort molecular techniques for dicistrovirus detection without rna extraction or purification
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/85477
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