Ethylene is involved in strawberry fruit ripening in an organ-specific manner
The fruit of the strawberry Fragaria×ananassa has traditionally been classified as non-climacteric because its ripening process is not governed by ethylene. However, previous studies have reported the timely endogenous production of minor amounts of ethylene by the fruit as well as the differential...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2013
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/85463 |
| Aporte de: |
| id |
I19-R120-10915-85463 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Botánica Ciencias Naturales Ethylene Fruit Metabolic profiling Non-climacteric Ripening Strawberry Etilenos Frutas Metabolismo Climaterio Maduración Frutillas |
| spellingShingle |
Botánica Ciencias Naturales Ethylene Fruit Metabolic profiling Non-climacteric Ripening Strawberry Etilenos Frutas Metabolismo Climaterio Maduración Frutillas Merchante, C. Vallarino, J. G. Osorio, S. Aragüez, I. Villarreal, N. Ariza, M. T. Martínez, Gustavo Adolfo Medina Escobar, N. Civello, Pedro Marcos Fernie, A. R. Botella, M. A. Valpuesta, V. Ethylene is involved in strawberry fruit ripening in an organ-specific manner |
| topic_facet |
Botánica Ciencias Naturales Ethylene Fruit Metabolic profiling Non-climacteric Ripening Strawberry Etilenos Frutas Metabolismo Climaterio Maduración Frutillas |
| description |
The fruit of the strawberry Fragaria×ananassa has traditionally been classified as non-climacteric because its ripening process is not governed by ethylene. However, previous studies have reported the timely endogenous production of minor amounts of ethylene by the fruit as well as the differential expression of genes of the ethylene synthesis, reception, and signalling pathways during fruit development. Mining of the Fragaria vesca genome allowed for the identification of the two main ethylene biosynthetic genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. Their expression pattern during fruit ripening was found to be stage and organ (achene or receptacle) specific. Strawberry plants with altered sensitivity to ethylene could be employed to unravel the role of ethylene in the ripening process of the strawberry fruit. To this end, independent lines of transgenic strawberry plants were generated that overexpress the Arabidopsis etr1-1 mutant ethylene receptor, which is a dominant negative allele, causing diminished sensitivity to ethylene. Genes involved in ethylene perception as well as in its related downstream processes, such as flavonoid biosynthesis, pectin metabolism, and volatile biosynthesis, were differently expressed in two transgenic tissues, the achene and the receptacle. The different transcriptional responsiveness of the achene and the receptacle to ethylene was also revealed by the metabolic profiling of the primary metabolites in these two organs. The free amino acid content was higher in the transgenic lines compared with the control in the mature achene, while glucose and fructose, and citric and malic acids were at lower levels. In the receptacle, the most conspicuous change in the transgenic lines was the depletion of the tricarboxylic acid cycle intermediates at the white stage of development, most probably as a consequence of diminished respiration. The results are discussed in the context of the importance of ethylene during strawberry fruit ripening. |
| format |
Articulo Articulo |
| author |
Merchante, C. Vallarino, J. G. Osorio, S. Aragüez, I. Villarreal, N. Ariza, M. T. Martínez, Gustavo Adolfo Medina Escobar, N. Civello, Pedro Marcos Fernie, A. R. Botella, M. A. Valpuesta, V. |
| author_facet |
Merchante, C. Vallarino, J. G. Osorio, S. Aragüez, I. Villarreal, N. Ariza, M. T. Martínez, Gustavo Adolfo Medina Escobar, N. Civello, Pedro Marcos Fernie, A. R. Botella, M. A. Valpuesta, V. |
| author_sort |
Merchante, C. |
| title |
Ethylene is involved in strawberry fruit ripening in an organ-specific manner |
| title_short |
Ethylene is involved in strawberry fruit ripening in an organ-specific manner |
| title_full |
Ethylene is involved in strawberry fruit ripening in an organ-specific manner |
| title_fullStr |
Ethylene is involved in strawberry fruit ripening in an organ-specific manner |
| title_full_unstemmed |
Ethylene is involved in strawberry fruit ripening in an organ-specific manner |
| title_sort |
ethylene is involved in strawberry fruit ripening in an organ-specific manner |
| publishDate |
2013 |
| url |
http://sedici.unlp.edu.ar/handle/10915/85463 |
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