Transgenic mouse model harboring the transcriptional fusion Ccl20-luciferase as a novel reporter of pro-inflammatory response

The chemokine CCL20, the unique ligand of CCR6 functions as an attractant of immune cells. Expression of CCL20 is induced by Toll-like Receptor (TLR) signaling or proinflammatory cytokine stimulation. However CCL20 is also constitutively produced at specific epithelial sites of mucosa. This expressi...

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Detalles Bibliográficos
Autores principales: Crispo, M., Van Maele, L., Tabareau, J., Cayet, D., Errea, Agustina Juliana, Ferreira, A. M., Rumbo, Martín, Sirard, J. C
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Química
Ciencias Exactas
Ccl20-Luciferase
Proinflammatory cytokine
Chemokines
Inmunología
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85454
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85454
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Ciencias Exactas
Ccl20-Luciferase
Proinflammatory cytokine
Chemokines
Inmunología
spellingShingle Química
Ciencias Exactas
Ccl20-Luciferase
Proinflammatory cytokine
Chemokines
Inmunología
Crispo, M.
Van Maele, L.
Tabareau, J.
Cayet, D.
Errea, Agustina Juliana
Ferreira, A. M.
Rumbo, Martín
Sirard, J. C
Transgenic mouse model harboring the transcriptional fusion Ccl20-luciferase as a novel reporter of pro-inflammatory response
topic_facet Química
Ciencias Exactas
Ccl20-Luciferase
Proinflammatory cytokine
Chemokines
Inmunología
description The chemokine CCL20, the unique ligand of CCR6 functions as an attractant of immune cells. Expression of CCL20 is induced by Toll-like Receptor (TLR) signaling or proinflammatory cytokine stimulation. However CCL20 is also constitutively produced at specific epithelial sites of mucosa. This expression profile is achieved by transcriptional regulation. In the present work we characterized regulatory features of mouse Ccl20 gene. Transcriptional fusions between the mouse Ccl20 promoter and the firefly luciferase (luc) encoding gene were constructed and assessed in in vitro and in vivo assays. We found that liver CCL20 expression and luciferase activity were upregulated by systemic administration of the TLR5 agonist flagellin. Using shRNA and dominant negative form specific for mouse TLR5, we showed that this expression was controlled by TLR5. To address in situ the regulation of gene activity, a transgenic mouse line harboring a functional Ccl20-luc fusion was generated. The luciferase expression was highly concordant with Ccl20 expression in different tissues. Our data indicate that the transgenic mouse model can be used to monitor activation of innate response in vivo.
format Articulo
Articulo
author Crispo, M.
Van Maele, L.
Tabareau, J.
Cayet, D.
Errea, Agustina Juliana
Ferreira, A. M.
Rumbo, Martín
Sirard, J. C
author_facet Crispo, M.
Van Maele, L.
Tabareau, J.
Cayet, D.
Errea, Agustina Juliana
Ferreira, A. M.
Rumbo, Martín
Sirard, J. C
author_sort Crispo, M.
title Transgenic mouse model harboring the transcriptional fusion Ccl20-luciferase as a novel reporter of pro-inflammatory response
title_short Transgenic mouse model harboring the transcriptional fusion Ccl20-luciferase as a novel reporter of pro-inflammatory response
title_full Transgenic mouse model harboring the transcriptional fusion Ccl20-luciferase as a novel reporter of pro-inflammatory response
title_fullStr Transgenic mouse model harboring the transcriptional fusion Ccl20-luciferase as a novel reporter of pro-inflammatory response
title_full_unstemmed Transgenic mouse model harboring the transcriptional fusion Ccl20-luciferase as a novel reporter of pro-inflammatory response
title_sort transgenic mouse model harboring the transcriptional fusion ccl20-luciferase as a novel reporter of pro-inflammatory response
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/85454
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bdutipo_str Repositorios
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