The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
Voltage-gated calcium channel (Cav) β subunits are auxiliary subunits to Cavs. Recent reports show Cavβ subunits may enter the nucleus and suggest a role in transcriptional regulation, but the physiological relevance of this localization remains unclear. We sought to define the nuclear function of C...
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| Autores principales: | , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2014
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/85405 |
| Aporte de: |
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I19-R120-10915-85405 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Médicas Química Cav, voltage-gated calcium channel ChIP, chromatin immunoprecipitation DM, differentiation medium EC, excitation- contraction EMSA, electrophoretic mobility shift assay GK, guanylate kinase GM, growth medium H&E, hematoxylin & eosin LMB, leptomycin-B MPC, muscle progenitor cell NC, noncanonical NLS, nuclear localization sequence RAd, recombinant adenoviral SH3, Src homology 3 TSS, transcription start site |
| spellingShingle |
Ciencias Médicas Química Cav, voltage-gated calcium channel ChIP, chromatin immunoprecipitation DM, differentiation medium EC, excitation- contraction EMSA, electrophoretic mobility shift assay GK, guanylate kinase GM, growth medium H&E, hematoxylin & eosin LMB, leptomycin-B MPC, muscle progenitor cell NC, noncanonical NLS, nuclear localization sequence RAd, recombinant adenoviral SH3, Src homology 3 TSS, transcription start site Taylor, J. Pereyra, Andrea Soledad Zhang, T. Messi, M. L. Wang, Z. M. Hereñú, Claudia Beatriz Kuan, P. F. Delbono, O. The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells |
| topic_facet |
Ciencias Médicas Química Cav, voltage-gated calcium channel ChIP, chromatin immunoprecipitation DM, differentiation medium EC, excitation- contraction EMSA, electrophoretic mobility shift assay GK, guanylate kinase GM, growth medium H&E, hematoxylin & eosin LMB, leptomycin-B MPC, muscle progenitor cell NC, noncanonical NLS, nuclear localization sequence RAd, recombinant adenoviral SH3, Src homology 3 TSS, transcription start site |
| description |
Voltage-gated calcium channel (Cav) β subunits are auxiliary subunits to Cavs. Recent reports show Cavβ subunits may enter the nucleus and suggest a role in transcriptional regulation, but the physiological relevance of this localization remains unclear. We sought to define the nuclear function of Cavβ in muscle progenitor cells (MPCs). We found that Cavβ1a is expressed in proliferating MPCs, before expression of the calcium conducting subunit Cav1.1, and enters the nucleus. Loss of Cavβ1a expression impaired MPC expansion in vitro and in vivo and caused widespread changes in global gene expression, including up-regulation of myogenin. Additionally, we found that Cavβ1a localizes to the promoter region of a number of genes, preferentially at noncanonical (NC) E-box sites. Cavβ1a binds to a region of the Myog promoter containing an NC E-box, suggesting a mechanism for inhibition of myogenin gene expression. This work indicates that Cavβ1a acts as a Cav-independent regulator of gene expression in MPCs, and is required for their normal expansion during myogenic development. |
| format |
Articulo Articulo |
| author |
Taylor, J. Pereyra, Andrea Soledad Zhang, T. Messi, M. L. Wang, Z. M. Hereñú, Claudia Beatriz Kuan, P. F. Delbono, O. |
| author_facet |
Taylor, J. Pereyra, Andrea Soledad Zhang, T. Messi, M. L. Wang, Z. M. Hereñú, Claudia Beatriz Kuan, P. F. Delbono, O. |
| author_sort |
Taylor, J. |
| title |
The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells |
| title_short |
The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells |
| title_full |
The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells |
| title_fullStr |
The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells |
| title_full_unstemmed |
The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells |
| title_sort |
ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells |
| publishDate |
2014 |
| url |
http://sedici.unlp.edu.ar/handle/10915/85405 |
| work_keys_str_mv |
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Repositorios |
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