rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer

We approached the identification of Ensifer (Sinorhizobium) meliloti conjugal functions by random Tn5-B13 mutagenesis of the pSmeLPU88a plasmid of E. meliloti strain LPU88 and the subsequent selection of those mutants that had lost the ability to mobilize the small plasmid pSmeLPU88b. The Tn5-B13-in...

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Detalles Bibliográficos
Autores principales:	Pistorio, Mariano, Torres Tejerizo, Gonzalo Arturo, Del Papa, María Florencia, Giusti, María de los Ángeles, Lozano, Mauricio Javier, Lagares, Antonio
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2013
Materias:	Ciencias Exactas Conjugation Ensifer meliloti Plasmid
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/85390
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-85390
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Conjugation Ensifer meliloti Plasmid
spellingShingle	Ciencias Exactas Conjugation Ensifer meliloti Plasmid Pistorio, Mariano Torres Tejerizo, Gonzalo Arturo Del Papa, María Florencia Giusti, María de los Ángeles Lozano, Mauricio Javier Lagares, Antonio rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
topic_facet	Ciencias Exactas Conjugation Ensifer meliloti Plasmid
description	We approached the identification of Ensifer (Sinorhizobium) meliloti conjugal functions by random Tn5-B13 mutagenesis of the pSmeLPU88a plasmid of E. meliloti strain LPU88 and the subsequent selection of those mutants that had lost the ability to mobilize the small plasmid pSmeLPU88b. The Tn5-B13-insertion site of one of the mutants was cloned as an EcoRI-restricted DNA fragment that after subsequent isolation and sequencing demonstrated that a small open reading frame of 522 bp (designated rptA, for rhizobium plasmid transfer A) had been disrupted. The predicted gene product encoded by the rptA sequence shows a significant similarity to two hypothetical proteins of the plasmid pSmed03 of Ensifer medicae WSM419 and other rhizobia plasmids. No significant similarity was found to any protein sequence of known function registered in the databases. Although the rptA gene was required for pSmeLPU88b-plasmid mobilization in the strain 2011 background, it was not required in the original strain LPU88 background.
format	Articulo Articulo
author	Pistorio, Mariano Torres Tejerizo, Gonzalo Arturo Del Papa, María Florencia Giusti, María de los Ángeles Lozano, Mauricio Javier Lagares, Antonio
author_facet	Pistorio, Mariano Torres Tejerizo, Gonzalo Arturo Del Papa, María Florencia Giusti, María de los Ángeles Lozano, Mauricio Javier Lagares, Antonio
author_sort	Pistorio, Mariano
title	rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title_short	rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title_full	rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title_fullStr	rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title_full_unstemmed	rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title_sort	rpta, a novel gene from ensifer (sinorhizobium) meliloti involved in conjugal transfer
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/85390
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bdutipo_str	Repositorios
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rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer

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