Effects of copper and/or cholesterol overload on mitochondrial function in a rat model of incipient neurodegeneration

Copper (Cu) and cholesterol (Cho) are both associated with neurodegenerative illnesses in humans and animals models. We studied the effect in Wistar rats of oral supplementation with trace amounts of Cu (3 ppm) and/or Cho (2%) in drinking water for 2 months. Increased amounts of nonceruloplasmin-bou...

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Autores principales: Arnal, Nathalie, Castillo, Omar, Tacconi de Alaniz, María Josefa, Marra, Carlos Alberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Médicas
Cobre
Colesterol
Neurodegeneración
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85290
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85290
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Cobre
Colesterol
Neurodegeneración
spellingShingle Ciencias Médicas
Cobre
Colesterol
Neurodegeneración
Arnal, Nathalie
Castillo, Omar
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
Effects of copper and/or cholesterol overload on mitochondrial function in a rat model of incipient neurodegeneration
topic_facet Ciencias Médicas
Cobre
Colesterol
Neurodegeneración
description Copper (Cu) and cholesterol (Cho) are both associated with neurodegenerative illnesses in humans and animals models. We studied the effect in Wistar rats of oral supplementation with trace amounts of Cu (3 ppm) and/or Cho (2%) in drinking water for 2 months. Increased amounts of nonceruloplasmin-bound Cu were observed in plasma and brain hippocampus together with a higher concentration of ceruloplasmin in plasma, cortex, and hippocampus. Cu, Cho, and the combined treatment Cu + Cho were able to induce a higher Cho/phospholipid ratio in mitochondrial membranes with a simultaneous decrease in glutathione content. The concentration of cardiolipin decreased and that of peroxidation products, conjugated dienes and lipoperoxides, increased. Treatments including Cho produced rigidization in both the outer and inner mitochondrial membranes with a simultaneous increase in permeability. No significant increase in Cyt C leakage to the cytosol was observed except in the case of cortex from rats treated with Cu and Cho nor were there any significant changes in caspase-3 activity and the Bax/Bcl2 ratio. However, the Aβ(1-42)/(1-40) ratio was higher in cortex and hippocampus. These findings suggest an incipient neurodegenerative process induced by Cu or Cho that might be potentiated by the association of the two supplements.
format Articulo
Articulo
author Arnal, Nathalie
Castillo, Omar
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
author_facet Arnal, Nathalie
Castillo, Omar
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
author_sort Arnal, Nathalie
title Effects of copper and/or cholesterol overload on mitochondrial function in a rat model of incipient neurodegeneration
title_short Effects of copper and/or cholesterol overload on mitochondrial function in a rat model of incipient neurodegeneration
title_full Effects of copper and/or cholesterol overload on mitochondrial function in a rat model of incipient neurodegeneration
title_fullStr Effects of copper and/or cholesterol overload on mitochondrial function in a rat model of incipient neurodegeneration
title_full_unstemmed Effects of copper and/or cholesterol overload on mitochondrial function in a rat model of incipient neurodegeneration
title_sort effects of copper and/or cholesterol overload on mitochondrial function in a rat model of incipient neurodegeneration
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/85290
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