Targeting a cross-reactive Gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model
<b>Background:</b> Cross-reactivity between soybean allergens and bovine caseins has been previously reported. In this study we aimed to map epitopes of the major soybean allergen Gly m 5 that are co-recognized by casein specific antibodies, and to identify a peptide responsible for the...
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| Autores principales: | , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2014
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/85280 |
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I19-R120-10915-85280 |
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dspace |
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Universidad Nacional de La Plata |
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I-19 |
| repository_str |
R-120 |
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SEDICI (UNLP) |
| language |
Inglés |
| topic |
Química Soybean allergen Cow's milk protein Milk allergy CMA patients |
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Química Soybean allergen Cow's milk protein Milk allergy CMA patients Curciarello, Renata Smaldini, Paola Lorena Candreva, Ángela María González, V. Parisi, Gustavo Daniel Cauerhff, Ana Albina Barrios, Ivana Analía Bruno Blanch, Luis Enrique Fossati, Carlos Alberto Petruccelli, Silvana Docena, Guillermo Horacio Targeting a cross-reactive Gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model |
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Química Soybean allergen Cow's milk protein Milk allergy CMA patients |
| description |
<b>Background:</b> Cross-reactivity between soybean allergens and bovine caseins has been previously reported. In this study we aimed to map epitopes of the major soybean allergen Gly m 5 that are co-recognized by casein specific antibodies, and to identify a peptide responsible for the cross-reactivity.
<b>Methods:</b> Cow's milk protein (CMP)-specific antibodies were used in different immunoassays (immunoblotting, ELISA, ELISA inhibition test) to evaluate the in vitro recognition of soybean proteins (SP). Recombinant Gly m 5 (α), a truncated fragment containing the C-terminal domain (α-T) and peptides of α-T were obtained and epitope mapping was performed with an overlapping peptide assay. Bioinformatics tools were used for epitope prediction by sequence alignment, and for modelling the cross-recognized soy proteins and peptides. The binding of SP to a monoclonal antibody was studied by surface Plasmon resonance (SPR). Finally, the in vivo cross-recognition of SP was assessed in a mouse model of milk allergy.
<b>Results:</b> Both α and α-T reacted with the different CMP-specific antibodies. α-T contains IgG and IgE epitopes in several peptides, particularly in the peptide named PA. Besides, we found similar values of association and dissociation constants between the α-casein specific mAb and the different milk and soy components. The food allergy mouse model showed that SP and PA contain the cross-reactive B and T epitopes, which triggered hypersensitivity reactions and a Th2-mediated response on CMP-sensitized mice.
<b>Conclusions:</b> Gly m 5 is a cross-reactive soy allergen and the α-T portion of the molecule contains IgG and IgE immunodominant epitopes, confined to PA, a region with enough conformation to be bound by antibodies. These findings contribute to explain the intolerance to SP observed in IgE-mediated CMA patients, primarily not sensitised to SP, as well as it sets the basis to propose a mucosal immunotherapy for milk allergy using this soy peptide. |
| format |
Articulo Articulo |
| author |
Curciarello, Renata Smaldini, Paola Lorena Candreva, Ángela María González, V. Parisi, Gustavo Daniel Cauerhff, Ana Albina Barrios, Ivana Analía Bruno Blanch, Luis Enrique Fossati, Carlos Alberto Petruccelli, Silvana Docena, Guillermo Horacio |
| author_facet |
Curciarello, Renata Smaldini, Paola Lorena Candreva, Ángela María González, V. Parisi, Gustavo Daniel Cauerhff, Ana Albina Barrios, Ivana Analía Bruno Blanch, Luis Enrique Fossati, Carlos Alberto Petruccelli, Silvana Docena, Guillermo Horacio |
| author_sort |
Curciarello, Renata |
| title |
Targeting a cross-reactive Gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model |
| title_short |
Targeting a cross-reactive Gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model |
| title_full |
Targeting a cross-reactive Gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model |
| title_fullStr |
Targeting a cross-reactive Gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model |
| title_full_unstemmed |
Targeting a cross-reactive Gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model |
| title_sort |
targeting a cross-reactive gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model |
| publishDate |
2014 |
| url |
http://sedici.unlp.edu.ar/handle/10915/85280 |
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