1H, 13C and 15N chemical shift assignments of Na-FAR-1, a helix-rich fatty acid and retinol binding protein of the parasitic nematode Necator americanus
The fatty acid and retinol-binding (FAR) proteins are a family of unusual helix-rich lipid binding proteins found exclusively in nematodes, and are secreted by a range of parasites of humans, animals and plants. Na-FAR-1 is from the parasitic nematode <i>Necator americanus</i>, an intest...
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| Autores principales: | , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2014
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/85270 |
| Aporte de: |
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I19-R120-10915-85270 |
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dspace |
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Universidad Nacional de La Plata |
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I-19 |
| repository_str |
R-120 |
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SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Médicas Fatty-acid and retinol-binding protein Na-FAR-1 Necator americanus NMR Parasitic nematode |
| spellingShingle |
Ciencias Médicas Fatty-acid and retinol-binding protein Na-FAR-1 Necator americanus NMR Parasitic nematode Rey Burusco, María Florencia Ibáñez Shimabukuro, Marina Cooper, A. Kennedy, Malcolm W. Córsico, Betina Smith, Brian O. 1H, 13C and 15N chemical shift assignments of Na-FAR-1, a helix-rich fatty acid and retinol binding protein of the parasitic nematode Necator americanus |
| topic_facet |
Ciencias Médicas Fatty-acid and retinol-binding protein Na-FAR-1 Necator americanus NMR Parasitic nematode |
| description |
The fatty acid and retinol-binding (FAR) proteins are a family of unusual helix-rich lipid binding proteins found exclusively in nematodes, and are secreted by a range of parasites of humans, animals and plants. Na-FAR-1 is from the parasitic nematode <i>Necator americanus</i>, an intestinal blood-feeding parasite of humans. Sequence-specific <SUP>1</SUP>H, <SUP>13</SUP>C and <SUP>15</SUP>N resonance assignments have been obtained for the recombinant 170 amino acid protein, using three-dimensional triple-resonance heteronuclear magnetic resonance experiments. Backbone assignments have been obtained for 99.3 % of the non-proline HN/N pairs (146 out of 147). The amide resonance of T45 was not observed, probably due to rapid exchange with solvent water. A total of 96.9 % of backbone resonances were identified, while 97.7 % assignment of amino acid sidechain protons is complete. All Hα(166), Hβ(250) and Hγ(160) and 98.4 % of the Hδ (126 out of 128) atoms were assigned. In addition, 99.4 % Cα (154 out of 155) and 99.3 % Cβ (143 out of 144) resonances have been assigned. No resonances were observed for the NH<SUB>n</SUB> groups of R93 N<SUB>ε</SUB>H<SUB>ε</SUB>, arginine, N<SUB>η1</SUB>H<SUB>2</SUB>, N<SUB>η2</SUB>H<SUB>2</SUB>, histidine N<SUB>δ1</SUB>H<SUB>δ1</SUB>, N<SUB>ε1</SUB>H<SUB>ε1</SUB> and lysine N<SUB>ζ3</SUB>H<SUB>3</SUB>. Na-FAR-1 has a similar overall arrangement of α-helices to Ce-FAR-7 of the free-living <i>Caeorhabditis elegans</i>, but with an extra C-terminal helix. |
| format |
Articulo Articulo |
| author |
Rey Burusco, María Florencia Ibáñez Shimabukuro, Marina Cooper, A. Kennedy, Malcolm W. Córsico, Betina Smith, Brian O. |
| author_facet |
Rey Burusco, María Florencia Ibáñez Shimabukuro, Marina Cooper, A. Kennedy, Malcolm W. Córsico, Betina Smith, Brian O. |
| author_sort |
Rey Burusco, María Florencia |
| title |
1H, 13C and 15N chemical shift assignments of Na-FAR-1, a helix-rich fatty acid and retinol binding protein of the parasitic nematode Necator americanus |
| title_short |
1H, 13C and 15N chemical shift assignments of Na-FAR-1, a helix-rich fatty acid and retinol binding protein of the parasitic nematode Necator americanus |
| title_full |
1H, 13C and 15N chemical shift assignments of Na-FAR-1, a helix-rich fatty acid and retinol binding protein of the parasitic nematode Necator americanus |
| title_fullStr |
1H, 13C and 15N chemical shift assignments of Na-FAR-1, a helix-rich fatty acid and retinol binding protein of the parasitic nematode Necator americanus |
| title_full_unstemmed |
1H, 13C and 15N chemical shift assignments of Na-FAR-1, a helix-rich fatty acid and retinol binding protein of the parasitic nematode Necator americanus |
| title_sort |
1h, 13c and 15n chemical shift assignments of na-far-1, a helix-rich fatty acid and retinol binding protein of the parasitic nematode necator americanus |
| publishDate |
2014 |
| url |
http://sedici.unlp.edu.ar/handle/10915/85270 |
| work_keys_str_mv |
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Repositorios |
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1764820489173204995 |