Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica

In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditiona...

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Autores principales:	Hiriart, Yanina, Serradell, María de los Ángeles, Martínez, Araci, Sampaolesi, Sofía, González Maciel, María Dolores, Chabalgoity, José Alejandro, Yim, Lucía, Algorta, Gabriela, Rumbo, Martín
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2013
Materias:	Salud Flagellin Monoclonal antibodies Salmonella Serotyping
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/85269
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-85269
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Salud Flagellin Monoclonal antibodies Salmonella Serotyping
spellingShingle	Salud Flagellin Monoclonal antibodies Salmonella Serotyping Hiriart, Yanina Serradell, María de los Ángeles Martínez, Araci Sampaolesi, Sofía González Maciel, María Dolores Chabalgoity, José Alejandro Yim, Lucía Algorta, Gabriela Rumbo, Martín Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
topic_facet	Salud Flagellin Monoclonal antibodies Salmonella Serotyping
description	In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health.
format	Articulo Articulo
author	Hiriart, Yanina Serradell, María de los Ángeles Martínez, Araci Sampaolesi, Sofía González Maciel, María Dolores Chabalgoity, José Alejandro Yim, Lucía Algorta, Gabriela Rumbo, Martín
author_facet	Hiriart, Yanina Serradell, María de los Ángeles Martínez, Araci Sampaolesi, Sofía González Maciel, María Dolores Chabalgoity, José Alejandro Yim, Lucía Algorta, Gabriela Rumbo, Martín
author_sort	Hiriart, Yanina
title	Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_short	Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_full	Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_fullStr	Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_full_unstemmed	Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica
title_sort	generation and selection of anti-flagellin monoclonal antibodies useful for serotyping salmonella enterica
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/85269
work_keys_str_mv	AT hiriartyanina generationandselectionofantiflagellinmonoclonalantibodiesusefulforserotypingsalmonellaenterica AT serradellmariadelosangeles generationandselectionofantiflagellinmonoclonalantibodiesusefulforserotypingsalmonellaenterica AT martinezaraci generationandselectionofantiflagellinmonoclonalantibodiesusefulforserotypingsalmonellaenterica AT sampaolesisofia generationandselectionofantiflagellinmonoclonalantibodiesusefulforserotypingsalmonellaenterica AT gonzalezmacielmariadolores generationandselectionofantiflagellinmonoclonalantibodiesusefulforserotypingsalmonellaenterica AT chabalgoityjosealejandro generationandselectionofantiflagellinmonoclonalantibodiesusefulforserotypingsalmonellaenterica AT yimlucia generationandselectionofantiflagellinmonoclonalantibodiesusefulforserotypingsalmonellaenterica AT algortagabriela generationandselectionofantiflagellinmonoclonalantibodiesusefulforserotypingsalmonellaenterica AT rumbomartin generationandselectionofantiflagellinmonoclonalantibodiesusefulforserotypingsalmonellaenterica
bdutipo_str	Repositorios
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Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica

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