Characterization of the Na+/HCO3¯ cotransport in human neutrophils

Background: Bicarbonate transport has crucial roles in regulating intracellular pH (pH<SUB>i</SUB>) in a variety of cells. The purpose of this study was to evaluate its participation in the regulation of pHi in resting and stimulated human neutrophils. Methods: Freshly isolated human neu...

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Autores principales: Giambelluca, Miriam Soledad, Ciancio, María Carolina, Orlowski, Alejandro, Gende, Oscar Alfredo, Pouliot, Marc, Aiello, Ernesto Alejandro
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Médicas
Blood cells
Intracellular pH
Ion transport
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85267
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85267
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Blood cells
Intracellular pH
Ion transport
spellingShingle Ciencias Médicas
Blood cells
Intracellular pH
Ion transport
Giambelluca, Miriam Soledad
Ciancio, María Carolina
Orlowski, Alejandro
Gende, Oscar Alfredo
Pouliot, Marc
Aiello, Ernesto Alejandro
Characterization of the Na+/HCO3¯ cotransport in human neutrophils
topic_facet Ciencias Médicas
Blood cells
Intracellular pH
Ion transport
description Background: Bicarbonate transport has crucial roles in regulating intracellular pH (pH<SUB>i</SUB>) in a variety of cells. The purpose of this study was to evaluate its participation in the regulation of pHi in resting and stimulated human neutrophils. Methods: Freshly isolated human neutrophils acidified by an ammonium prepulse were used in this study. Results: We demonstrated that resting neutrophils have a bicarbonate transport mechanism that prevents acidification when the Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger is blocked by EIPA. Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na<SUP>+</SUP>/HCO<SUB>3</SUB>¯ cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. In western blot and RT-PCR analysis the expression of NBCe2 but not NBCe1 or NBCn1 was detected in neutrophils Acidified neutrophils increased the EIPA-insensitive pHi recovery rate when its activity was stimulated with fMLF/cytochalasin B. This increase in the removal of acid equivalents was insensitive to the blockade of the NADPH oxidase with DPI. Conclusion: It is concluded that neutrophils have an NBC that regulates basal pH<SUB>i</SUB> and is modulated by chemotactic agents.
format Articulo
Articulo
author Giambelluca, Miriam Soledad
Ciancio, María Carolina
Orlowski, Alejandro
Gende, Oscar Alfredo
Pouliot, Marc
Aiello, Ernesto Alejandro
author_facet Giambelluca, Miriam Soledad
Ciancio, María Carolina
Orlowski, Alejandro
Gende, Oscar Alfredo
Pouliot, Marc
Aiello, Ernesto Alejandro
author_sort Giambelluca, Miriam Soledad
title Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title_short Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title_full Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title_fullStr Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title_full_unstemmed Characterization of the Na+/HCO3¯ cotransport in human neutrophils
title_sort characterization of the na+/hco3¯ cotransport in human neutrophils
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85267
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