Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors

Among sequenced genomes, it is common to find a high proportion of genes encoding proteins that cannot be assigned a known function. In bacterial genomes, genes related to a similar function are often located in contiguous regions. The presence of genes encoding conserved hypothetical proteins (chp)...

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Autores principales: López Fuentes, Eunice, Torres Tejerizo, Gonzalo Arturo, Cervantes, Laura, Brom, Susana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Exactas
Conjugation
Hypothetical proteins
Quorum-sensing
Rhizobia
Transcriptional regulation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85260
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85260
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Conjugation
Hypothetical proteins
Quorum-sensing
Rhizobia
Transcriptional regulation
spellingShingle Ciencias Exactas
Conjugation
Hypothetical proteins
Quorum-sensing
Rhizobia
Transcriptional regulation
López Fuentes, Eunice
Torres Tejerizo, Gonzalo Arturo
Cervantes, Laura
Brom, Susana
Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors
topic_facet Ciencias Exactas
Conjugation
Hypothetical proteins
Quorum-sensing
Rhizobia
Transcriptional regulation
description Among sequenced genomes, it is common to find a high proportion of genes encoding proteins that cannot be assigned a known function. In bacterial genomes, genes related to a similar function are often located in contiguous regions. The presence of genes encoding conserved hypothetical proteins (chp) in such a region may suggest that they are related to that particular function. Plasmid pRet42a from <i>Rhizobium etli</i> CFN42 is a conjugative plasmid containing a segment of approximately 30 Kb encoding genes involved in conjugative transfer. In addition to genes responsible for Dtr (DNA transfer and replication), Mpf (Mating pair formation) and regulation, it has two chp-encoding genes (RHE_PA00163 and RHE_PA00164) and a transcriptional regulator (RHE_PA00165). RHE_PA00163 encodes an uncharacterized protein conserved in bacteria that presents a COG4634 conserved domain, and RHE_PA00164 encodes an uncharacterized conserved protein with a DUF433 domain of unknown function. RHE_PA00165 presents a HTH_XRE domain, characteristic of DNA-binding proteins belonging to the xenobiotic response element family of transcriptional regulators. Interestingly, genes similar to these are also present in transfer regions of plasmids from other bacteria. To determine if these genes participate in conjugative transfer, we mutagenized them and analyzed their conjugative phenotype. A mutant in RHE_PA00163 showed a slight (10 times) but reproducible increase in transfer frequency from <i>Rhizobium</i> donors, while mutants in RHE_PA00164 and RHE_PA00165 lost their ability to transfer the plasmid from some <i>Agrobacterium</i> donors. Our results indicate that the chp-encoding genes located among conjugation genes are indeed related to this function. However, the participation of RHE_PA00164 and RHE_PA00165 is only revealed under very specific circumstances, and is not perceived when the plasmid is transferred from the original host. RHE_PA00163 seems to be a fine-tuning modulator for conjugative transfer.
format Articulo
Articulo
author López Fuentes, Eunice
Torres Tejerizo, Gonzalo Arturo
Cervantes, Laura
Brom, Susana
author_facet López Fuentes, Eunice
Torres Tejerizo, Gonzalo Arturo
Cervantes, Laura
Brom, Susana
author_sort López Fuentes, Eunice
title Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors
title_short Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors
title_full Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors
title_fullStr Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors
title_full_unstemmed Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors
title_sort genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pret42a from rhizobium etli cfn42 participate in modulating transfer and affect conjugation from different donors
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85260
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