N-Nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes

This study was conducted to explore how the nature of the acyl chains of sphingomyelin (SM) influence its lateral distribution in the ternary lipid mixture SM/cholesterol/1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), focusing on the importance of the hydrophobic part of the SM molecule for domain...

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Detalles Bibliográficos
Autores principales: Maté, Sabina María, Busto, J. V., García-Arribas, A.B., Sot, J., Vázquez, Romina Florencia, Herlax, Vanesa Silvana, Wolf, C., Bakás, Laura Susana, Goñi, F. M.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Bioquímica
Biología
N-Nervonoylsphingomyelin
Colesterol
Sphingomyelin
Estructura molecular
Membrana Celular
Biofísica
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85226
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85226
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Biología
N-Nervonoylsphingomyelin
Colesterol
Sphingomyelin
Estructura molecular
Membrana Celular
Biofísica
spellingShingle Bioquímica
Biología
N-Nervonoylsphingomyelin
Colesterol
Sphingomyelin
Estructura molecular
Membrana Celular
Biofísica
Maté, Sabina María
Busto, J. V.
García-Arribas, A.B.
Sot, J.
Vázquez, Romina Florencia
Herlax, Vanesa Silvana
Wolf, C.
Bakás, Laura Susana
Goñi, F. M.
N-Nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes
topic_facet Bioquímica
Biología
N-Nervonoylsphingomyelin
Colesterol
Sphingomyelin
Estructura molecular
Membrana Celular
Biofísica
description This study was conducted to explore how the nature of the acyl chains of sphingomyelin (SM) influence its lateral distribution in the ternary lipid mixture SM/cholesterol/1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), focusing on the importance of the hydrophobic part of the SM molecule for domain formation. Atomic force microscopy (AFM) measurements showed that the presence of a double bond in the 24:1 SM molecule in mixtures with cholesterol (CHO) or in pure bilayers led to a decrease in the molecular packing. Confocal microscopy and AFM showed, at the meso- and nanoscales respectively, that unlike 16:0 and 24:0 SM, 24:1 SM does not induce phase segregation in ternary lipid mixtures with DOPC and CHO. This ternary lipid mixture had a nanomechanical stability intermediate between those displayed by liquid-ordered (Lo) and liquid-disordered (Ld) phases, as reported by AFM force spectroscopy measurements, demonstrating that 24:1 SM is able to accommodate both DOPC and CHO, forming a single phase. Confocal experiments on giant unilamellar vesicles made of human, sheep, and rabbit erythrocyte ghosts rich in 24:1 SM and CHO, showed no lateral domain segregation. This study provides insights into how the specific molecular structure of SM affects the lateral behavior and the physical properties of both model and natural membranes. Specifically, the data suggest that unsaturated SM may help to keep membrane lipids in a homogeneous mixture rather than in separate domains.
format Articulo
Articulo
author Maté, Sabina María
Busto, J. V.
García-Arribas, A.B.
Sot, J.
Vázquez, Romina Florencia
Herlax, Vanesa Silvana
Wolf, C.
Bakás, Laura Susana
Goñi, F. M.
author_facet Maté, Sabina María
Busto, J. V.
García-Arribas, A.B.
Sot, J.
Vázquez, Romina Florencia
Herlax, Vanesa Silvana
Wolf, C.
Bakás, Laura Susana
Goñi, F. M.
author_sort Maté, Sabina María
title N-Nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes
title_short N-Nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes
title_full N-Nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes
title_fullStr N-Nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes
title_full_unstemmed N-Nervonoylsphingomyelin (C24:1) prevents lateral heterogeneity in cholesterol-containing membranes
title_sort n-nervonoylsphingomyelin (c24:1) prevents lateral heterogeneity in cholesterol-containing membranes
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85226
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