Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows

The objectives of this study were to assess the agreement between endometrial cytology and uterine biopsy for the diagnosis of subclinical endometritis (SEND) in grazing dairy cows, the interobserver agreement of the biopsy's readings, and the bacterial population isolated from the uterus of co...

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Autores principales: Madoz, Laura Vanina, Giuliodori, Mauricio Javier, Migliorisi, Ana Lorena, Jaureguiberry, María, Sota, Rodolfo Luzbel de la
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Veterinarias
Bacteriology
Dairy cow
Subclinical endometritis
Uterine biopsy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85191
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85191
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Bacteriology
Dairy cow
Subclinical endometritis
Uterine biopsy
spellingShingle Ciencias Veterinarias
Bacteriology
Dairy cow
Subclinical endometritis
Uterine biopsy
Madoz, Laura Vanina
Giuliodori, Mauricio Javier
Migliorisi, Ana Lorena
Jaureguiberry, María
Sota, Rodolfo Luzbel de la
Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows
topic_facet Ciencias Veterinarias
Bacteriology
Dairy cow
Subclinical endometritis
Uterine biopsy
description The objectives of this study were to assess the agreement between endometrial cytology and uterine biopsy for the diagnosis of subclinical endometritis (SEND) in grazing dairy cows, the interobserver agreement of the biopsy's readings, and the bacterial population isolated from the uterus of cows having SEND. In experiment 1, lactating Holstein cows (n. = 44) 31 to 59. d in milk (DIM) at sampling were enrolled. Clinical endometritis was diagnosed by direct evaluation of vaginal discharge and SEND by endometrial cytology evaluation. Two hundred cells per smear were counted to determine the percentage of polymorphonuclear neutrophilic leukocytes (PMNL). Cut-off values used were ≥8% PMNL at ≤33 DIM, ≥6% PMNL at 34 to 47 DIM, and ≥4% PMNL at ≥48 DIM. Biopsies were assessed blindly by 2 observers who categorized them into 4 groups according to their inflammatory changes: none, minimal, moderate, and severe inflammatory changes. Data were analyzed using the kappa coefficient and logistic regression. In experiment 2, lactating Holstein cows (n. = 60) 21 to 62 DIM were enrolled. Clinical endometritis and SEND were diagnosed as previously described. Samples were cultured for aerobic and anaerobic bacteria by routine methods of bacteriological testing. Data were analyzed with logistic regression. In experiment 1, little agreement was observed between cytology and biopsy outputs (kappa. = 0.151), and strong agreement between the 2 operators (kappa. = 0.854). The likelihood of having a normal biopsy (no inflammatory change) was greater for healthy cows than for those having SEND (odds ratio. = 13.145). The probability for getting normal uterine tissue decreased 2.1% for every increasing percentage point in PMNL. In experiment 2, no bacteria were isolated from cows with SEND, coagulase-negative staphylococci were commonly isolated from healthy cows, and <i>Trueperella pyogenes</i> was frequently isolated from cows with clinical endometritis. The likelihood of isolating <i>T. pyogenes</i> from uterine samples increased with the percentage of PMNL (odds ratio. = 1.100). In conclusion, biopsy showed low agreement with cytology for the diagnosis of SEND. Nevertheless, fertility trials using uterine biopsies to predict pregnancy outcomes are needed to determine its diagnostic usefulness. Finally, bacteriology would not be recommended as a diagnostic tool because no bacteria were isolated from cows with SEND.
format Articulo
Articulo
author Madoz, Laura Vanina
Giuliodori, Mauricio Javier
Migliorisi, Ana Lorena
Jaureguiberry, María
Sota, Rodolfo Luzbel de la
author_facet Madoz, Laura Vanina
Giuliodori, Mauricio Javier
Migliorisi, Ana Lorena
Jaureguiberry, María
Sota, Rodolfo Luzbel de la
author_sort Madoz, Laura Vanina
title Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows
title_short Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows
title_full Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows
title_fullStr Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows
title_full_unstemmed Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows
title_sort endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85191
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