Relationship between impaired adipogenesis of retroperitoneal adipose tissue and hypertrophic obesity: role of endogenous glucocorticoid excess

Although the pro-adipogenic effect of glucocorticoid (GC) on adipose tissue (AT) precursor cell differentiation is openly accepted, the effect of chronically high peripheral levels of GC on AT mass expansion is not fully understood. In the present study, we aim to assess the <i>in vitro</i&...

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Autores principales: Zubiría, María Guillermina, Vidal Bravo, Juana, Spinedi, Eduardo Julio, Giovambattista, Andrés
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Exactas
Ciencias Médicas
Adipokines
ADX
Cell lipid
HRT
MSG rat
Pro-/anti-adipogenic signals
SVF cells
Visceral adiposity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85155
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85155
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Ciencias Médicas
Adipokines
ADX
Cell lipid
HRT
MSG rat
Pro-/anti-adipogenic signals
SVF cells
Visceral adiposity
spellingShingle Ciencias Exactas
Ciencias Médicas
Adipokines
ADX
Cell lipid
HRT
MSG rat
Pro-/anti-adipogenic signals
SVF cells
Visceral adiposity
Zubiría, María Guillermina
Vidal Bravo, Juana
Spinedi, Eduardo Julio
Giovambattista, Andrés
Relationship between impaired adipogenesis of retroperitoneal adipose tissue and hypertrophic obesity: role of endogenous glucocorticoid excess
topic_facet Ciencias Exactas
Ciencias Médicas
Adipokines
ADX
Cell lipid
HRT
MSG rat
Pro-/anti-adipogenic signals
SVF cells
Visceral adiposity
description Although the pro-adipogenic effect of glucocorticoid (GC) on adipose tissue (AT) precursor cell differentiation is openly accepted, the effect of chronically high peripheral levels of GC on AT mass expansion is not fully understood. In the present study, we aim to assess the <i>in vitro</i> adipogenic capacity of AT precursor cells isolated from retroperitoneal (RP) AT pads of the hypercorticosteronaemic, adult neonatally treated monosodium L-glutamate (MSG) male rat. To ascertain this issue, we explored the <i>in vitro</i> adipogenic process of stromal-vascular fraction (SVF) cells isolated from RPAT pads of 60-day-old MSG rats. The data recorded indicated that RPAT-SVF cells from hypercorticosteronaemic MSG rats, although displaying an enhanced proliferation capacity, differentiated slower than normal cells. This dysfunction was associated with a reduction in key parameters indicative of precursor cell commitment, differentiation capacity and the percentage of fully differentiated adipocytes, with a retarded maturation process. The distorted adipogenic capacity was highly conditioned by RPAT-SVF cells displaying a low committed population and both excessive and reduced expression of anti- (Pref-1 and Wnt-10b) and pro-adipogenic (mineralocorticoid receptor) signals respectively. Notably, the normalization of peripheral corticosterone levels in MSG rats, as a result of bilateral adrenalectomy combined with GC replacement therapy, fully prevented reduced RPAT precursor cell commitment and overall impaired adipogenesis. Our study strongly supports that the impaired adipogenic process observed in the adult hypertrophic obese MSG male rat is a GC-dependent mechanism, thus explaining the unhealthy RPAT expansion observed in human hypertrophic obese phenotypes, such as in the Cushing's syndrome.
format Articulo
Articulo
author Zubiría, María Guillermina
Vidal Bravo, Juana
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_facet Zubiría, María Guillermina
Vidal Bravo, Juana
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_sort Zubiría, María Guillermina
title Relationship between impaired adipogenesis of retroperitoneal adipose tissue and hypertrophic obesity: role of endogenous glucocorticoid excess
title_short Relationship between impaired adipogenesis of retroperitoneal adipose tissue and hypertrophic obesity: role of endogenous glucocorticoid excess
title_full Relationship between impaired adipogenesis of retroperitoneal adipose tissue and hypertrophic obesity: role of endogenous glucocorticoid excess
title_fullStr Relationship between impaired adipogenesis of retroperitoneal adipose tissue and hypertrophic obesity: role of endogenous glucocorticoid excess
title_full_unstemmed Relationship between impaired adipogenesis of retroperitoneal adipose tissue and hypertrophic obesity: role of endogenous glucocorticoid excess
title_sort relationship between impaired adipogenesis of retroperitoneal adipose tissue and hypertrophic obesity: role of endogenous glucocorticoid excess
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85155
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AT spinedieduardojulio relationshipbetweenimpairedadipogenesisofretroperitonealadiposetissueandhypertrophicobesityroleofendogenousglucocorticoidexcess
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bdutipo_str Repositorios
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