Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases

Experimental evidence has suggested that plasma membrane (PM)-associated signaling and hence cell metabolism and viability depend on lipid composition and organization. The aim of the present work is to develop a cell model to study the endogenous polyunsaturated fatty acids (PUFAs) effect on PM pro...

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Detalles Bibliográficos
Autores principales: Jaureguiberry, M. Soledad, Tricerri, María Alejandra, Sánchez, Susana A., Finarelli, Gabriela Sandra, Montanaro, Mauro Aldo, Prieto, Eduardo Daniel, Rimoldi, Omar Jorge
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Bioquímica
cholesterol transport
cytotoxicity
fatty acid desaturases
human apolipoprotein A-I
membrane heterogeneity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85151
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85151
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
cholesterol transport
cytotoxicity
fatty acid desaturases
human apolipoprotein A-I
membrane heterogeneity
spellingShingle Bioquímica
cholesterol transport
cytotoxicity
fatty acid desaturases
human apolipoprotein A-I
membrane heterogeneity
Jaureguiberry, M. Soledad
Tricerri, María Alejandra
Sánchez, Susana A.
Finarelli, Gabriela Sandra
Montanaro, Mauro Aldo
Prieto, Eduardo Daniel
Rimoldi, Omar Jorge
Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases
topic_facet Bioquímica
cholesterol transport
cytotoxicity
fatty acid desaturases
human apolipoprotein A-I
membrane heterogeneity
description Experimental evidence has suggested that plasma membrane (PM)-associated signaling and hence cell metabolism and viability depend on lipid composition and organization. The aim of the present work is to develop a cell model to study the endogenous polyunsaturated fatty acids (PUFAs) effect on PM properties and analyze its influence on cholesterol (Chol) homeostasis. We have previously shown that by using a cell line over-expressing stearoyl-CoA-desaturase, membrane composition and organization coordinate cellular pathways involved in Chol efflux and cell viability by different mechanisms. Now, we expanded our studies to a cell model over-expressing both Δ5 and Δ6 desaturases, which resulted in a permanently higher PUFA content in PM. Furthermore, this cell line showed increased PM fluidity, Chol storage, and mitochondrial activity. In addition, human apolipoprotein A-I-mediated Chol removal was less efficient in these cells than in the corresponding control. Taken together, our results suggested that the cell functionality is preserved by regulating PM organization and Chol exportation and homeostasis.
format Articulo
Articulo
author Jaureguiberry, M. Soledad
Tricerri, María Alejandra
Sánchez, Susana A.
Finarelli, Gabriela Sandra
Montanaro, Mauro Aldo
Prieto, Eduardo Daniel
Rimoldi, Omar Jorge
author_facet Jaureguiberry, M. Soledad
Tricerri, María Alejandra
Sánchez, Susana A.
Finarelli, Gabriela Sandra
Montanaro, Mauro Aldo
Prieto, Eduardo Daniel
Rimoldi, Omar Jorge
author_sort Jaureguiberry, M. Soledad
title Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases
title_short Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases
title_full Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases
title_fullStr Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases
title_full_unstemmed Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases
title_sort role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85151
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