De novo fatty acid synthesis at the mitotic exit is required to complete cellular division

Although the regulation of the cell cycle has been extensively studied, much less is known about its coordination with the cellular metabolism. Using mass spectrometry we found that lysophospholipid levels decreased drastically from G2/M to G1 phase, while de novo phosphatidylcholine synthesis, the...

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Autores principales:	Scaglia, Natalia, Tyekucheva, Svitlana, Zadra, Giorgia, Photopoulos, Cornelia, Loda, Massimo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2014
Materias:	Ciencias Médicas AMPK C75 Cell cycle Cell cycle arrest De novo lipogenesis Fatty acid Lysophospholipid Metabolome Phospholipid
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/85132
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-85132
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas AMPK C75 Cell cycle Cell cycle arrest De novo lipogenesis Fatty acid Lysophospholipid Metabolome Phospholipid
spellingShingle	Ciencias Médicas AMPK C75 Cell cycle Cell cycle arrest De novo lipogenesis Fatty acid Lysophospholipid Metabolome Phospholipid Scaglia, Natalia Tyekucheva, Svitlana Zadra, Giorgia Photopoulos, Cornelia Loda, Massimo De novo fatty acid synthesis at the mitotic exit is required to complete cellular division
topic_facet	Ciencias Médicas AMPK C75 Cell cycle Cell cycle arrest De novo lipogenesis Fatty acid Lysophospholipid Metabolome Phospholipid
description	Although the regulation of the cell cycle has been extensively studied, much less is known about its coordination with the cellular metabolism. Using mass spectrometry we found that lysophospholipid levels decreased drastically from G2/M to G1 phase, while de novo phosphatidylcholine synthesis, the main phospholipid in mammalian cells, increased, suggesting that enhanced membrane production was concomitant to a decrease in its turnover. In addition, fatty acid synthesis and incorporation into membranes was increased upon cell division. The rate-limiting reaction for de novo fatty acid synthesis is catalyzed by acetyl-CoA carboxylase. As expected, its inhibiting phosphorylation decreased prior to cytokinesis initiation. Importantly, the inhibition of fatty acid synthesis arrested the cells at G2/M despite the presence of abundant fatty acids in the media. Our results suggest that de novo lipogenesis is essential for cell cycle completion. This "lipogenic checkpoint" at G2/M may be therapeutically exploited for hyperproliferative diseases such as cancer.
format	Articulo Articulo
author	Scaglia, Natalia Tyekucheva, Svitlana Zadra, Giorgia Photopoulos, Cornelia Loda, Massimo
author_facet	Scaglia, Natalia Tyekucheva, Svitlana Zadra, Giorgia Photopoulos, Cornelia Loda, Massimo
author_sort	Scaglia, Natalia
title	De novo fatty acid synthesis at the mitotic exit is required to complete cellular division
title_short	De novo fatty acid synthesis at the mitotic exit is required to complete cellular division
title_full	De novo fatty acid synthesis at the mitotic exit is required to complete cellular division
title_fullStr	De novo fatty acid synthesis at the mitotic exit is required to complete cellular division
title_full_unstemmed	De novo fatty acid synthesis at the mitotic exit is required to complete cellular division
title_sort	de novo fatty acid synthesis at the mitotic exit is required to complete cellular division
publishDate	2014
url	http://sedici.unlp.edu.ar/handle/10915/85132
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De novo fatty acid synthesis at the mitotic exit is required to complete cellular division

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