Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis

Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolate...

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Autores principales: Gornatti Churria, Carlos Daniel, Vigo, Germán Blas, Origlia, Javier Aníbal, Campos, Josefina, Caffer, María, Píscopo, Miguel Víctor, Herrero Loyola, Miguel Ángel, Petruccelli, Miguel Ángel, Pichel, Mariana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Veterinarias
Chinchilla lanigera
Farmed chinchillas
PFGE
Salmonella Typhimurium
Zoonosis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85125
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85125
record_format dspace
spelling I19-R120-10915-851252024-04-24T18:35:59Z http://sedici.unlp.edu.ar/handle/10915/85125 Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis Diagnóstico de un brote de Salmonella Typhimurium en chinchillas (Chinchilla lanigera) mediante la electroforesis en gel de campo pulsado Gornatti Churria, Carlos Daniel Vigo, Germán Blas Origlia, Javier Aníbal Campos, Josefina Caffer, María Píscopo, Miguel Víctor Herrero Loyola, Miguel Ángel Petruccelli, Miguel Ángel Pichel, Mariana 2014 2019-11-07T14:32:46Z en Ciencias Veterinarias Chinchilla lanigera Farmed chinchillas PFGE Salmonella Typhimurium Zoonosis Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys and intestines from each of the five animals evaluated. The five strains were susceptible to ampicillin, cephalotin, cefotaxime, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, nitrofurantoin and trimethoprimsulfamethoxazole, and resistant to tetracycline. Each of the five S. Typhimurium isolates was analyzed by XbaI-pulsed-field gel electrophoresis (PFGE), showing an identical electrophoretic profile with 15 defined bands, which was found to be identical to pattern ARJPXX01.0220 of the PulseNet Argentine National database of Salmonella PFGE patterns. This is the first work describing the postmortem diagnosis of an outbreak of salmonellosis in chinchillas by using molecular methods such as PFGE. Empleando estudios anatomopatológicos y microbiológicos se examinó a un grupo de chinchillas (Chinchilla lanigera) adultas que murieron súbitamente en 2012 en una granja de la ciudad de La Plata (Buenos Aires, Argentina). Se aisló Salmonella enterica serovar Typhimurium (S. Typhimurium) del hígado, el bazo, el corazón, los pulmones, los riñones y los intestinos de los cinco animales evaluados. Los cinco aislamientos estudiados (uno por animal) fueron sensibles a ampicilina, cefalotina, cefotaxima, ácido nalidíxico, gentamicina, estreptomicina, cloranfenicol, fosfomicina, nitrofurantoína y trimetoprima-sulfametoxazol, y resistentes a tetraciclina. El análisis de dichos aislamientos por electroforesis en gel de campo pulsado [pulsed-fi eld gel electrophoresis (PFGE)] con XbaI mostró un perÀ l electroforético idéntico con 15 bandas, idéntico a su vez al patrón ARJPXX01.0220 del banco nacional argentino de datos de PulseNet, que cuenta con patrones de PFGE de Salmonella. El presente trabajo describe por primera vez el diagnóstico postmortem de un brote de salmonelosis en chinchillas usando un método molecular, como la electroforesis en gel en campo pulsado. Facultad de Ciencias Veterinarias Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 205-209
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Chinchilla lanigera
Farmed chinchillas
PFGE
Salmonella Typhimurium
Zoonosis
spellingShingle Ciencias Veterinarias
Chinchilla lanigera
Farmed chinchillas
PFGE
Salmonella Typhimurium
Zoonosis
Gornatti Churria, Carlos Daniel
Vigo, Germán Blas
Origlia, Javier Aníbal
Campos, Josefina
Caffer, María
Píscopo, Miguel Víctor
Herrero Loyola, Miguel Ángel
Petruccelli, Miguel Ángel
Pichel, Mariana
Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
topic_facet Ciencias Veterinarias
Chinchilla lanigera
Farmed chinchillas
PFGE
Salmonella Typhimurium
Zoonosis
description Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys and intestines from each of the five animals evaluated. The five strains were susceptible to ampicillin, cephalotin, cefotaxime, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, nitrofurantoin and trimethoprimsulfamethoxazole, and resistant to tetracycline. Each of the five S. Typhimurium isolates was analyzed by XbaI-pulsed-field gel electrophoresis (PFGE), showing an identical electrophoretic profile with 15 defined bands, which was found to be identical to pattern ARJPXX01.0220 of the PulseNet Argentine National database of Salmonella PFGE patterns. This is the first work describing the postmortem diagnosis of an outbreak of salmonellosis in chinchillas by using molecular methods such as PFGE.
format Articulo
Articulo
author Gornatti Churria, Carlos Daniel
Vigo, Germán Blas
Origlia, Javier Aníbal
Campos, Josefina
Caffer, María
Píscopo, Miguel Víctor
Herrero Loyola, Miguel Ángel
Petruccelli, Miguel Ángel
Pichel, Mariana
author_facet Gornatti Churria, Carlos Daniel
Vigo, Germán Blas
Origlia, Javier Aníbal
Campos, Josefina
Caffer, María
Píscopo, Miguel Víctor
Herrero Loyola, Miguel Ángel
Petruccelli, Miguel Ángel
Pichel, Mariana
author_sort Gornatti Churria, Carlos Daniel
title Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
title_short Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
title_full Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
title_fullStr Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
title_full_unstemmed Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
title_sort diagnosis of an outbreak of salmonella typhimurium in chinchillas (chinchilla lanigera) by pulsed-field gel electrophoresis
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85125
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