Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the...
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Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
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2014
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/85064 |
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I19-R120-10915-85064 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Ciencias Exactas IncP-1 IncP-7 IncP-9 Environmental Habitats Hot Spots |
spellingShingle |
Ciencias Exactas IncP-1 IncP-7 IncP-9 Environmental Habitats Hot Spots Dealtry, Simone Ding, Guo Chun Weichelt, Viola Dunon, Vincent Schlüter, Andreas Martini, María Carla Del Papa, María Florencia Lagares, Antonio Amos, Gregory Charles Auton Wellington, Elizabeth Margaret Helen Gaze, William Hugo Sipkema, Detmer Sjöling, Sara Springael, Dirk Heuer, Holger Elsas, Jan Dirk van Thomas, Christopher Smalla, Kornelia Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
topic_facet |
Ciencias Exactas IncP-1 IncP-7 IncP-9 Environmental Habitats Hot Spots |
description |
IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the <i>rep</i> gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are "hot spots" of plasmids potentially carrying catabolic genes. |
format |
Articulo Articulo |
author |
Dealtry, Simone Ding, Guo Chun Weichelt, Viola Dunon, Vincent Schlüter, Andreas Martini, María Carla Del Papa, María Florencia Lagares, Antonio Amos, Gregory Charles Auton Wellington, Elizabeth Margaret Helen Gaze, William Hugo Sipkema, Detmer Sjöling, Sara Springael, Dirk Heuer, Holger Elsas, Jan Dirk van Thomas, Christopher Smalla, Kornelia |
author_facet |
Dealtry, Simone Ding, Guo Chun Weichelt, Viola Dunon, Vincent Schlüter, Andreas Martini, María Carla Del Papa, María Florencia Lagares, Antonio Amos, Gregory Charles Auton Wellington, Elizabeth Margaret Helen Gaze, William Hugo Sipkema, Detmer Sjöling, Sara Springael, Dirk Heuer, Holger Elsas, Jan Dirk van Thomas, Christopher Smalla, Kornelia |
author_sort |
Dealtry, Simone |
title |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
title_short |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
title_full |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
title_fullStr |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
title_full_unstemmed |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
title_sort |
cultivation-independent screening revealed hot spots of incp-1, incp-7 and incp-9 plasmid occurrence in different environmental habitats |
publishDate |
2014 |
url |
http://sedici.unlp.edu.ar/handle/10915/85064 |
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