Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats

IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the...

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Autores principales: Dealtry, Simone, Ding, Guo Chun, Weichelt, Viola, Dunon, Vincent, Schlüter, Andreas, Martini, María Carla, Del Papa, María Florencia, Lagares, Antonio, Amos, Gregory Charles Auton, Wellington, Elizabeth Margaret Helen, Gaze, William Hugo, Sipkema, Detmer, Sjöling, Sara, Springael, Dirk, Heuer, Holger, Elsas, Jan Dirk van, Thomas, Christopher, Smalla, Kornelia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85064
Aporte de:
id I19-R120-10915-85064
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
IncP-1
IncP-7
IncP-9
Environmental Habitats
Hot Spots
spellingShingle Ciencias Exactas
IncP-1
IncP-7
IncP-9
Environmental Habitats
Hot Spots
Dealtry, Simone
Ding, Guo Chun
Weichelt, Viola
Dunon, Vincent
Schlüter, Andreas
Martini, María Carla
Del Papa, María Florencia
Lagares, Antonio
Amos, Gregory Charles Auton
Wellington, Elizabeth Margaret Helen
Gaze, William Hugo
Sipkema, Detmer
Sjöling, Sara
Springael, Dirk
Heuer, Holger
Elsas, Jan Dirk van
Thomas, Christopher
Smalla, Kornelia
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
topic_facet Ciencias Exactas
IncP-1
IncP-7
IncP-9
Environmental Habitats
Hot Spots
description IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the <i>rep</i> gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are "hot spots" of plasmids potentially carrying catabolic genes.
format Articulo
Articulo
author Dealtry, Simone
Ding, Guo Chun
Weichelt, Viola
Dunon, Vincent
Schlüter, Andreas
Martini, María Carla
Del Papa, María Florencia
Lagares, Antonio
Amos, Gregory Charles Auton
Wellington, Elizabeth Margaret Helen
Gaze, William Hugo
Sipkema, Detmer
Sjöling, Sara
Springael, Dirk
Heuer, Holger
Elsas, Jan Dirk van
Thomas, Christopher
Smalla, Kornelia
author_facet Dealtry, Simone
Ding, Guo Chun
Weichelt, Viola
Dunon, Vincent
Schlüter, Andreas
Martini, María Carla
Del Papa, María Florencia
Lagares, Antonio
Amos, Gregory Charles Auton
Wellington, Elizabeth Margaret Helen
Gaze, William Hugo
Sipkema, Detmer
Sjöling, Sara
Springael, Dirk
Heuer, Holger
Elsas, Jan Dirk van
Thomas, Christopher
Smalla, Kornelia
author_sort Dealtry, Simone
title Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title_short Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title_full Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title_fullStr Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title_full_unstemmed Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title_sort cultivation-independent screening revealed hot spots of incp-1, incp-7 and incp-9 plasmid occurrence in different environmental habitats
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85064
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