Plasma-mediated inactivation of Pseudomonas aeruginosa biofilms grown on borosilicate surfaces under continuous culture system

Biofilms are microbial communities attached to a surface and embedded in a matrix composed of exopolysaccharides and excreted nucleic acids. Bacterial biofilms are responsible for undesirable effects such as disease, prostheses colonization, biofouling, equipment damage, and pipe plugging. Biofilms...

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Detalles Bibliográficos
Autores principales: Vandervoort, Kurt, Brelles Mariño, Graciela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Biología
Biofilms
Gas discharge plasmas
Continuous system
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85000
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85000
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Biofilms
Gas discharge plasmas
Continuous system
spellingShingle Biología
Biofilms
Gas discharge plasmas
Continuous system
Vandervoort, Kurt
Brelles Mariño, Graciela
Plasma-mediated inactivation of Pseudomonas aeruginosa biofilms grown on borosilicate surfaces under continuous culture system
topic_facet Biología
Biofilms
Gas discharge plasmas
Continuous system
description Biofilms are microbial communities attached to a surface and embedded in a matrix composed of exopolysaccharides and excreted nucleic acids. Bacterial biofilms are responsible for undesirable effects such as disease, prostheses colonization, biofouling, equipment damage, and pipe plugging. Biofilms are also more resilient than free-living cells to regular sterilization methods and therefore it is indispensable to develop better ways to control and remove them. The use of gas discharge plasmas is a good alternative since plasmas contain a mixture of reactive agents well-known for their decontamination potential against free microorganisms. We have previously reported that Pseudomonas aeruginosa biofilms were inactivated after a 1-min plasma exposure. We determined that the adhesiveness and the thickness of Pseudomonas biofilms grown on borosilicate were reduced. We also reported sequential morphological changes and loss of viability upon plasma treatment. However, the studies were carried out in batch cultures. The use of a continuous culture results in a more homogenous environment ensuring reproducible biofilm growth. The aim of this work was to study plasma-mediated inactivation of P. aeruginosa biofilms grown on borosilicate in a continuous culture system. In this paper we show that biofilms grown on glass under continuous culture can be inactivated by using gas discharge plasma. Both biofilm architecture and cell culturabilty are impacted by the plasma treatment. The inactivation kinetics is similar to previously described ones and cells go through sequential changes ranging from minimal modification without loss of viability at short plasma exposure times, to major structure and viability loss at longer exposure times. We report that changes in biofilm structure leading to the loss of culturability and viability are related to a decrease of the biofilm matrix adhesiveness. To our knowledge, there has been no attempt to evaluate the inactivation/sterilization of biofilms grown in a continuous system.
format Articulo
Articulo
author Vandervoort, Kurt
Brelles Mariño, Graciela
author_facet Vandervoort, Kurt
Brelles Mariño, Graciela
author_sort Vandervoort, Kurt
title Plasma-mediated inactivation of Pseudomonas aeruginosa biofilms grown on borosilicate surfaces under continuous culture system
title_short Plasma-mediated inactivation of Pseudomonas aeruginosa biofilms grown on borosilicate surfaces under continuous culture system
title_full Plasma-mediated inactivation of Pseudomonas aeruginosa biofilms grown on borosilicate surfaces under continuous culture system
title_fullStr Plasma-mediated inactivation of Pseudomonas aeruginosa biofilms grown on borosilicate surfaces under continuous culture system
title_full_unstemmed Plasma-mediated inactivation of Pseudomonas aeruginosa biofilms grown on borosilicate surfaces under continuous culture system
title_sort plasma-mediated inactivation of pseudomonas aeruginosa biofilms grown on borosilicate surfaces under continuous culture system
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85000
work_keys_str_mv AT vandervoortkurt plasmamediatedinactivationofpseudomonasaeruginosabiofilmsgrownonborosilicatesurfacesundercontinuousculturesystem
AT brellesmarinograciela plasmamediatedinactivationofpseudomonasaeruginosabiofilmsgrownonborosilicatesurfacesundercontinuousculturesystem
bdutipo_str Repositorios
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