The equine arteritis virus isolate from the 2010 Argentinian outbreak

A semen sample from a stallion infected during the 2010 equine arteritis virus (EAV) outbreak was received for viral isolation prior to castration of the animal. The virus was identified using a polyclonal antibody immunofluorescence test. Reverse-transcription polymerase chain reaction (RT-PCR) was...

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Autores principales: Metz, Germán Ernesto, Serena, María Soledad, Panei, Carlos Javier, Nosetto, Edgardo Omar, Echeverría, María Gabriela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Veterinarias
Argentina
Equine arteritis virus
Outbreak
Semen sample
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84993
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84993
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Argentina
Equine arteritis virus
Outbreak
Semen sample
spellingShingle Ciencias Veterinarias
Argentina
Equine arteritis virus
Outbreak
Semen sample
Metz, Germán Ernesto
Serena, María Soledad
Panei, Carlos Javier
Nosetto, Edgardo Omar
Echeverría, María Gabriela
The equine arteritis virus isolate from the 2010 Argentinian outbreak
topic_facet Ciencias Veterinarias
Argentina
Equine arteritis virus
Outbreak
Semen sample
description A semen sample from a stallion infected during the 2010 equine arteritis virus (EAV) outbreak was received for viral isolation prior to castration of the animal. The virus was identified using a polyclonal antibody immunofluorescence test. Reverse-transcription polymerase chain reaction (RT-PCR) was used to amplify a region of the GP5 gene with primers GL105F and GL673R. The PCR products were purified and sequences of both strands were determined in a MegaBACE™ 1000 with inner primers CR2 and EAV32. A phylogenetic datasetwas built with the previously reported sequences of five strains isolated in Argentina, together with a group of selected sequences obtained from GenBank. The unrooted neighbour- joining tree was constructed using molecular evolutionary genetic analysis (MEGA) and bootstrap analyses were conducted using 1,000 replicate datasets. Evolutionary distances were computed using the maximum composite likelihood method. A NetNGIyc server analysis at the Technical University of Denmark (www.cbs.dtu.dk/services/NetNGIyc/) was used to predict N-glycosylation in GP5 sequences. The phylogenetic analysis revealed that the new strain (GLD-LP-ARG), together with other strains previously isolated, belongs to the European group EU-1 but in a different branch. The new strain shows 99% nucleotide identity with strain A1 and 98.1% with the Belgian strain 08P178. Persistently infected stallions and their cryopreserved semen constitute a reservoir of EAV, which ensures its persistence in the horse population around the world. These findings reinforce the importance of careful monitoring of persistently infected stallions, as well as semen straws, by RT-PCR ortest mating, in accordance with national regulations.
format Articulo
Articulo
author Metz, Germán Ernesto
Serena, María Soledad
Panei, Carlos Javier
Nosetto, Edgardo Omar
Echeverría, María Gabriela
author_facet Metz, Germán Ernesto
Serena, María Soledad
Panei, Carlos Javier
Nosetto, Edgardo Omar
Echeverría, María Gabriela
author_sort Metz, Germán Ernesto
title The equine arteritis virus isolate from the 2010 Argentinian outbreak
title_short The equine arteritis virus isolate from the 2010 Argentinian outbreak
title_full The equine arteritis virus isolate from the 2010 Argentinian outbreak
title_fullStr The equine arteritis virus isolate from the 2010 Argentinian outbreak
title_full_unstemmed The equine arteritis virus isolate from the 2010 Argentinian outbreak
title_sort equine arteritis virus isolate from the 2010 argentinian outbreak
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/84993
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bdutipo_str Repositorios
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