Formation of 4-keto-D-aldopentoses and 4-pentulosonates (4-keto-D-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria

In our previous study, a new microbial reaction yielding 4-keto-D-arabonate from 2,5-diketo-D-gluconate was identified with Gluconacetobacter liquefaciens RCTMR 10. It appeared that decarboxylation and dehydrogenation took place together in the reaction. To analyze the nature of the reaction, invest...

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Detalles Bibliográficos
Autores principales: Adachi, Osao, Hours, Roque Alberto, Shinagawa, Emiko, Akakabe, Yoshihiko, Yakushi, Toshiharu, Matsushita, Kazunobu
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Química
2,5-diketo-D-gluconate decarboxylase
4-keto-D-aldopentose
4-keto-Daldopentose 1-dehydrogenase
4-keto-Dpentonate
D-aldopentose 4-dehydrogenase
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84937
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84937
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
2,5-diketo-D-gluconate decarboxylase
4-keto-D-aldopentose
4-keto-Daldopentose 1-dehydrogenase
4-keto-Dpentonate
D-aldopentose 4-dehydrogenase
spellingShingle Química
2,5-diketo-D-gluconate decarboxylase
4-keto-D-aldopentose
4-keto-Daldopentose 1-dehydrogenase
4-keto-Dpentonate
D-aldopentose 4-dehydrogenase
Adachi, Osao
Hours, Roque Alberto
Shinagawa, Emiko
Akakabe, Yoshihiko
Yakushi, Toshiharu
Matsushita, Kazunobu
Formation of 4-keto-D-aldopentoses and 4-pentulosonates (4-keto-D-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria
topic_facet Química
2,5-diketo-D-gluconate decarboxylase
4-keto-D-aldopentose
4-keto-Daldopentose 1-dehydrogenase
4-keto-Dpentonate
D-aldopentose 4-dehydrogenase
description In our previous study, a new microbial reaction yielding 4-keto-D-arabonate from 2,5-diketo-D-gluconate was identified with Gluconacetobacter liquefaciens RCTMR 10. It appeared that decarboxylation and dehydrogenation took place together in the reaction. To analyze the nature of the reaction, investigations were done with the membrane fraction of the organism, and 4-keto-D-arabinose was confirmed as the direct precursor of 4-keto-D-arabonate. Two novel membrane-bound enzymes, 2,5-diketo-D-gluconate decarboxylase and 4-keto-D-aldopentose 1-dehydrogenase, were involved in the reaction. Alternatively, D-arabonate was oxidized to 4-keto-D-arabonate by another membrane-bound enzyme, D-arabonate 4-dehydrogenase. More directly, D-arabinose oxidation was examined with growing cells and with the membrane fraction of G. suboxydans IFO 12528. 4-Keto-D-arabinose, the same intermediate as that from 2,5-diketo-D-gluconate, was detected, and it was oxidized to 4-keto-D-arabonate. Likewise, D-ribose was oxidized to 4-keto-D-ribose and then it was oxidized to 4-keto-D-ribonate. In addition to 4-keto-D-aldopentose 1-dehydrogenase, the presence of a novel membranebound enzyme, D-aldopentose 4-dehydrogenase, was confirmed in the membrane fraction. The formation of 4-keto-D-aldopentoses and 4-keto-D-pentonates (4-pentulosonates) was finally confirmed as reaction products of four different novel membrane-bound enzymes.
format Articulo
Articulo
author Adachi, Osao
Hours, Roque Alberto
Shinagawa, Emiko
Akakabe, Yoshihiko
Yakushi, Toshiharu
Matsushita, Kazunobu
author_facet Adachi, Osao
Hours, Roque Alberto
Shinagawa, Emiko
Akakabe, Yoshihiko
Yakushi, Toshiharu
Matsushita, Kazunobu
author_sort Adachi, Osao
title Formation of 4-keto-D-aldopentoses and 4-pentulosonates (4-keto-D-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria
title_short Formation of 4-keto-D-aldopentoses and 4-pentulosonates (4-keto-D-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria
title_full Formation of 4-keto-D-aldopentoses and 4-pentulosonates (4-keto-D-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria
title_fullStr Formation of 4-keto-D-aldopentoses and 4-pentulosonates (4-keto-D-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria
title_full_unstemmed Formation of 4-keto-D-aldopentoses and 4-pentulosonates (4-keto-D-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria
title_sort formation of 4-keto-d-aldopentoses and 4-pentulosonates (4-keto-d-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/84937
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