Expression of islet neogenesis-associated protein in islets of normal hamsters

The aim of the present study was to test the possible presence and expression of islet neogenesis-associated protein (INGAP) in islet cells of normal adult hamsters. Pancreata from normal male Syrian hamsters were removed to perform the following studies. (i) Western blot analysis using the cytosoli...

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Autores principales: Flores, Luis Emilio, García, María Elisa, Borelli, María Inés, Del Zotto, Héctor Herminio, Alzugaray, María Eugenia, Maiztegui, Bárbara, Gagliardino, Juan José
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Ciencias Médicas
Proteína
INGAP
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84806
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84806
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Proteína
INGAP
spellingShingle Ciencias Médicas
Proteína
INGAP
Flores, Luis Emilio
García, María Elisa
Borelli, María Inés
Del Zotto, Héctor Herminio
Alzugaray, María Eugenia
Maiztegui, Bárbara
Gagliardino, Juan José
Expression of islet neogenesis-associated protein in islets of normal hamsters
topic_facet Ciencias Médicas
Proteína
INGAP
description The aim of the present study was to test the possible presence and expression of islet neogenesis-associated protein (INGAP) in islet cells of normal adult hamsters. Pancreata from normal male Syrian hamsters were removed to perform the following studies. (i) Western blot analysis using the cytosolic fraction from homogenates of isolated islets, exocrine tissue and whole pancreas, and rabbit INGAP-specific antibody. (ii) Immunohistochemical identification of INGAP-positive cells in fixed sections of intact pancreata, fresh and 72 h cultured islets (isolated by collagenase digestion), and smears of exocrine pancreatic cells, using the same INGAP-specific antibody and streptavidin-biotin complex. (iii) RT-PCR using total RNA extracted from isolated islets and from exocrine tissue as template, and a specific pair of primers. (iv) Control of the sequence of the PCR products. INGAP protein was identified by Western blot in the cytosolic fraction of homogenates from fresh isolated islets, exocrine cells and whole fresh pancreas. INGAP-immunopositive cells were observed in duct, exocrine and islet cells in either fixed intact or digested pancreatic tissue. INGAP mRNA was identified in samples of total RNA from fresh and cultured isolated islets and from exocrine cells. Our data demonstrate that INGAP is present and expressed in islets and in exocrine pancreatic cells of normal hamsters. The ubiquitous localization of INGAP suggests its possible role in the physiological process of islet growth and its protective effect upon streptozotocin-induced diabetes.
format Articulo
Articulo
author Flores, Luis Emilio
García, María Elisa
Borelli, María Inés
Del Zotto, Héctor Herminio
Alzugaray, María Eugenia
Maiztegui, Bárbara
Gagliardino, Juan José
author_facet Flores, Luis Emilio
García, María Elisa
Borelli, María Inés
Del Zotto, Héctor Herminio
Alzugaray, María Eugenia
Maiztegui, Bárbara
Gagliardino, Juan José
author_sort Flores, Luis Emilio
title Expression of islet neogenesis-associated protein in islets of normal hamsters
title_short Expression of islet neogenesis-associated protein in islets of normal hamsters
title_full Expression of islet neogenesis-associated protein in islets of normal hamsters
title_fullStr Expression of islet neogenesis-associated protein in islets of normal hamsters
title_full_unstemmed Expression of islet neogenesis-associated protein in islets of normal hamsters
title_sort expression of islet neogenesis-associated protein in islets of normal hamsters
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/84806
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