Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells

The in vitro effect of the antioxidant α-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progressio...

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Autores principales: Soloneski, Sonia María Elsa, Reigosa, Miguel A., Larramendy, Marcelo Luis
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Ciencias Naturales
Vitamina E
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84783
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84783
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Vitamina E
spellingShingle Ciencias Naturales
Vitamina E
Soloneski, Sonia María Elsa
Reigosa, Miguel A.
Larramendy, Marcelo Luis
Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells
topic_facet Ciencias Naturales
Vitamina E
description The in vitro effect of the antioxidant α-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progression and mitotic index (MI) as genetic end points. Both zineb and azzurro activities were tested within the range 0.1-100.0 μg/ml on exponentially growing CHO cells preincubated for 24 h in the presence or absence of 50.0 μg/ml vitamin E. SCE frequencies increased significantly over control values in a concentration-dependent manner in zineb- and azzurro-treated cultures at concentrations of 0.1-10.0 and 0.1-25.0 μg/ml, respectively. When target cells were preincubated with vitamin E, the number of SCEs was significantly lower than that observed in cells exposed only to 1.0-10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro, but higher than control values. Cytotoxicity was observed at concentrations higher than 25.0 and 50.0 μg/ml zineb and azzurro, respectively, regardless of the absence or presence of vitamin E. Regression analysis showed that the proliferative rate index decreased as a function of the concentration of zineb (0.1-10.0 μg/ml concentration range) and azzurro (0.1-25.0 μg/ml concentration range) titrated into cultures. For both chemicals, progressive concentration-related inhibition of the mitotic activity from cultures was observed when 10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro was employed. However, no significant alteration in cell cycle progression or MI was observed between vitamin E-preincubated cultures and those treated only with zineb and azzurro.
format Articulo
Articulo
author Soloneski, Sonia María Elsa
Reigosa, Miguel A.
Larramendy, Marcelo Luis
author_facet Soloneski, Sonia María Elsa
Reigosa, Miguel A.
Larramendy, Marcelo Luis
author_sort Soloneski, Sonia María Elsa
title Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells
title_short Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells
title_full Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells
title_fullStr Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells
title_full_unstemmed Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells
title_sort vitamin e prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in chinese hamster ovary cells
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/84783
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AT larramendymarceloluis vitaminepreventsethylenebisdithiocarbamatepesticidezinebinducedsisterchromatidexchangeinchinesehamsterovarycells
bdutipo_str Repositorios
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