Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium

Objective: The aim was to examine the regulation of the cardiac Na<SUP>+</SUP>-independent Cl¯/HCO<SUB>3</SUB>¯ exchanger (AE) mRNA isoform expression in association to the enhanced AE activity in the hypertrophied myocardium of spontaneously hypertensive rats (SHR). Methods:...

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Autores principales: Chiappe de Cingolani, Gladys Ethel, Morgan, Patricio Eduardo, Mundiña-Weilenmann, Cecilia, Casey, Joseph, Fujinaga, Jocelyne, Camilión de Hurtado, María Cristina, Cingolani, Horacio Eugenio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Ciencias Médicas
Gene expression
Hypertension
Hypertrophy
Ion exchangers
Membrane transport
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84718
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84718
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Gene expression
Hypertension
Hypertrophy
Ion exchangers
Membrane transport
spellingShingle Ciencias Médicas
Gene expression
Hypertension
Hypertrophy
Ion exchangers
Membrane transport
Chiappe de Cingolani, Gladys Ethel
Morgan, Patricio Eduardo
Mundiña-Weilenmann, Cecilia
Casey, Joseph
Fujinaga, Jocelyne
Camilión de Hurtado, María Cristina
Cingolani, Horacio Eugenio
Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
topic_facet Ciencias Médicas
Gene expression
Hypertension
Hypertrophy
Ion exchangers
Membrane transport
description Objective: The aim was to examine the regulation of the cardiac Na<SUP>+</SUP>-independent Cl¯/HCO<SUB>3</SUB>¯ exchanger (AE) mRNA isoform expression in association to the enhanced AE activity in the hypertrophied myocardium of spontaneously hypertensive rats (SHR). Methods: AE activity was determined by the initial rates of the pH<SUB>i</SUB> recovery from imposed intracellular alkalinization (forward mode of exchange) and the pH<SUB>i</SUB> rise induced by Cl¯ removal (reverse mode). Net HCO<SUB>3</SUB>¯ (J<sub>HCO<SUB>3</SUB>¯</sub>) efflux and influx were respectively determined. AE mRNA isoforms were analyzed by Northern blot with specific probes to detect AE1, AE2 and AE3 mRNAs. Results: Initial J<sub>HCO<SUB>3</SUB>¯</sub> efflux after imposed alkaline load (pH<SUB>i</SUB>≅7.5) was higher in SHR than in normotensive WKY rats (3.01±0.33, n=7, vs. 0.64±0.29 mM/min, n=5, P<0.05). J<sub>HCO<SUB>3</SUB>¯</sub> influx induced by Cl¯ deprivation was also increased in SHR, 4.24±0.56 mM/min (n=10) versus 2.31±0.26 (n=10, P<0.05) in WKY. In arbitrary units, the 4.1-kb AE1 mRNA decreased in SHR (0.15±0.01, n=7) compared to WKY (0.29±0.06, n=7, P<0.05), whereas the 3.6-kb mRNA did not change. AE2 mRNAs were similarly expressed in WKY and SHR. Cardiac specific AE3 (cAE3) mRNA decreased in SHR, 1.10±0.16 arbitrary units (n=8) versus 1.79±0.24, (n=8, P<0.05) in WKY. Full length AE3 (flAE3) mRNA increased from 0.69±0.06 (WKY, n=8) to 1.25±0.19 arbitrary units in SHR (n=8, P<0.05). Conclusions: The increase in flAE3 mRNA expression in cardiac tissue from the SHR is an adaptive change of the hypertrophied myocardium that might be in connection with the increased activity of the AE.
format Articulo
Articulo
author Chiappe de Cingolani, Gladys Ethel
Morgan, Patricio Eduardo
Mundiña-Weilenmann, Cecilia
Casey, Joseph
Fujinaga, Jocelyne
Camilión de Hurtado, María Cristina
Cingolani, Horacio Eugenio
author_facet Chiappe de Cingolani, Gladys Ethel
Morgan, Patricio Eduardo
Mundiña-Weilenmann, Cecilia
Casey, Joseph
Fujinaga, Jocelyne
Camilión de Hurtado, María Cristina
Cingolani, Horacio Eugenio
author_sort Chiappe de Cingolani, Gladys Ethel
title Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title_short Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title_full Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title_fullStr Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title_full_unstemmed Hyperactivity and altered mRNA isoform expression of the Cl¯/HCO3¯ anion-exchanger in the hypertrophied myocardium
title_sort hyperactivity and altered mrna isoform expression of the cl¯/hco3¯ anion-exchanger in the hypertrophied myocardium
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/84718
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