Mechanisms involved in the β-cell mass increase induced by chronic sucrose feeding to normal rats

The aim of the present study was to clarify the mechanisms by which a sucrose-rich diet (SRD) produces an increase in the pancreatic β-cell mass in the rat. Normal Wistar rats were fed for 30 weeks either an SRD (SRD rats; 63% wt/wt), or the same diet but with starch instead of sucrose in the same p...

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Detalles Bibliográficos
Autores principales: Del Zotto, Héctor Herminio, Gómez Dumm, César Leandro Alberto, Drago, S., Fortino, A., Luna, Georgina Cecilia, Gagliardino, Juan José
Formato: Articulo
Lenguaje:Inglés
Publicado: 2002
Materias:
Ciencias Médicas
β-cell mass
rats
sucrose-rich diet
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84641
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84641
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
β-cell mass
rats
sucrose-rich diet
spellingShingle Ciencias Médicas
β-cell mass
rats
sucrose-rich diet
Del Zotto, Héctor Herminio
Gómez Dumm, César Leandro Alberto
Drago, S.
Fortino, A.
Luna, Georgina Cecilia
Gagliardino, Juan José
Mechanisms involved in the β-cell mass increase induced by chronic sucrose feeding to normal rats
topic_facet Ciencias Médicas
β-cell mass
rats
sucrose-rich diet
description The aim of the present study was to clarify the mechanisms by which a sucrose-rich diet (SRD) produces an increase in the pancreatic β-cell mass in the rat. Normal Wistar rats were fed for 30 weeks either an SRD (SRD rats; 63% wt/wt), or the same diet but with starch instead of sucrose in the same proportion (CD rats). We studied body weight, serum glucose and triacylglycerol levels, endocrine tissue and β-cell mass, β-cell replication rate (proliferating cell nuclear antigen; PCNA), islet neogenesis (cytokeratin immunostaining) and β-cell apoptosis (propidium iodide). Body weight (g) recorded in the SRD rats was significantly (P<0.05) larger than that of the CD group (556.0 ± 8.3 vs 470.0 ± 13.1). Both serum glucose and triacylglycerol levels (mmol/l) were also significantly higher (P<0.05) in SRD than in CD rats (serum glucose, 8.11 ± 0.14 vs 6.62 ± 0.17; triacyglycerol, 1.57 ± 0.18 vs 0.47 ± 0.04). The number of pancreatic islets per unit area increased significantly (P<0.05) in SRD rats (3.29 ± 0.1 vs 2.01 ± 0.2). A significant increment (2.6 times) in the mass of endocrine tissue was detected in SRD animals, mainly due to an increase in the β-cell mass (P=0.0025). The islet cell replication rate, measured as the percentage of PCNA-labelled β cells increased 6.8 times in SRD rats (P<0.03). The number of apoptotic cells in the endocrine pancreas decreased significantly (three times) in the SRD animals (P=0.03). The cytokeratin-positive area did not show significant differences between CD and SRD rats. The increase of β-cell mass induced by SRD was accomplished by an enhanced replication of β cells together with a decrease in the rate of β-cell apoptosis, without any evident participation of islet neogenesis. This pancreatic reaction was unable to maintain serum glucose levels of these rats at the level measured in CD animals.
format Articulo
Articulo
author Del Zotto, Héctor Herminio
Gómez Dumm, César Leandro Alberto
Drago, S.
Fortino, A.
Luna, Georgina Cecilia
Gagliardino, Juan José
author_facet Del Zotto, Héctor Herminio
Gómez Dumm, César Leandro Alberto
Drago, S.
Fortino, A.
Luna, Georgina Cecilia
Gagliardino, Juan José
author_sort Del Zotto, Héctor Herminio
title Mechanisms involved in the β-cell mass increase induced by chronic sucrose feeding to normal rats
title_short Mechanisms involved in the β-cell mass increase induced by chronic sucrose feeding to normal rats
title_full Mechanisms involved in the β-cell mass increase induced by chronic sucrose feeding to normal rats
title_fullStr Mechanisms involved in the β-cell mass increase induced by chronic sucrose feeding to normal rats
title_full_unstemmed Mechanisms involved in the β-cell mass increase induced by chronic sucrose feeding to normal rats
title_sort mechanisms involved in the β-cell mass increase induced by chronic sucrose feeding to normal rats
publishDate 2002
url http://sedici.unlp.edu.ar/handle/10915/84641
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