Patterns of MUC1 tissue expression defined by an Anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer

Our aim was to determine the pattern of expression of MUC1 mucin cytoplasmic tail (MUC1 CT) in breast carcinoma. A total of 98 invasive breast adenocarcinoma tumor samples were assayed by immunohistochemical (IHC) analysis. The pattern of reaction was classified as membrane, cytoplasmic, or mixed. S...

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Autores principales: Croce, María Virginia, Isla Larrain, Marina Teresita, Rua, Carina E., Rabassa, Martín Enrique, Gendler, Sandra J., Segal-Eiras, Amada
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Ciencias Médicas
Breast cancer
Immunohistochemical study
MUC 1 cytoplasmic tail
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84583
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84583
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Breast cancer
Immunohistochemical study
MUC 1 cytoplasmic tail
spellingShingle Ciencias Médicas
Breast cancer
Immunohistochemical study
MUC 1 cytoplasmic tail
Croce, María Virginia
Isla Larrain, Marina Teresita
Rua, Carina E.
Rabassa, Martín Enrique
Gendler, Sandra J.
Segal-Eiras, Amada
Patterns of MUC1 tissue expression defined by an Anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer
topic_facet Ciencias Médicas
Breast cancer
Immunohistochemical study
MUC 1 cytoplasmic tail
description Our aim was to determine the pattern of expression of MUC1 mucin cytoplasmic tail (MUC1 CT) in breast carcinoma. A total of 98 invasive breast adenocarcinoma tumor samples were assayed by immunohistochemical (IHC) analysis. The pattern of reaction was classified as membrane, cytoplasmic, or mixed. Subcellular fractions were prepared after SDS-PAGE and Western blotting. The antibodies employed were anti-MUC1 CT (CT2 monoclonal antibody, MAb) and C595 MAb against the extracellular MUC1 core protein. With the CT2 MAb, IHC showed a high percentage of positive staining in 93% of specimens, with membrane staining the most common pattern observed. C595 MAb was reactive in 73% of specimens. Similar percentages of membrane and cytoplasmic staining were found, mainly in a mixed pattern. Western blotting showed different bands. With the CT2 MAb, the membrane fraction showed the most intense reaction; a strong band of reaction was detected at approximately <30 kD. With the C595 MAb, in most cases a double band at 200 kD was found. In breast epithelium, the pattern of MUC1 CT expression may constitute an indicator of MUC1 production because it does not depend on glycosylation. The pattern and extension of MUC1 CT positivity do not vary according to the histopathological subtype of the tumor.
format Articulo
Articulo
author Croce, María Virginia
Isla Larrain, Marina Teresita
Rua, Carina E.
Rabassa, Martín Enrique
Gendler, Sandra J.
Segal-Eiras, Amada
author_facet Croce, María Virginia
Isla Larrain, Marina Teresita
Rua, Carina E.
Rabassa, Martín Enrique
Gendler, Sandra J.
Segal-Eiras, Amada
author_sort Croce, María Virginia
title Patterns of MUC1 tissue expression defined by an Anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer
title_short Patterns of MUC1 tissue expression defined by an Anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer
title_full Patterns of MUC1 tissue expression defined by an Anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer
title_fullStr Patterns of MUC1 tissue expression defined by an Anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer
title_full_unstemmed Patterns of MUC1 tissue expression defined by an Anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer
title_sort patterns of muc1 tissue expression defined by an anti-muc1 cytoplasmic tail monoclonal antibody in breast cancer
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/84583
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