Strain selection for improvement of Bradyrhizobium japonicum competitiveness for nodulation of soybean

A Bradyrhizobium japonicum USDA 110-derived strain able to produce wider halos in soft-agar medium than its parental strain was obtained by recurrent selection. It was more chemotactic than the wild type towards mannitol and three amino acids. When cultured in minimal medium with mannitol as a singl...

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Detalles Bibliográficos
Autores principales: Althabegoiti, María Julia, López García, Silvina Laura, Piccinetti, Carlos, Mongiardini, Elías Javier, Pérez Giménez, Julieta, Quelas, Juan Ignacio, Perticari, Alejandro, Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Biología
Bradyrhizobium
Nodule occupation
Soybean
Strain selection
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84576
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84576
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bradyrhizobium
Nodule occupation
Soybean
Strain selection
spellingShingle Biología
Bradyrhizobium
Nodule occupation
Soybean
Strain selection
Althabegoiti, María Julia
López García, Silvina Laura
Piccinetti, Carlos
Mongiardini, Elías Javier
Pérez Giménez, Julieta
Quelas, Juan Ignacio
Perticari, Alejandro
Lodeiro, Aníbal Roberto
Strain selection for improvement of Bradyrhizobium japonicum competitiveness for nodulation of soybean
topic_facet Biología
Bradyrhizobium
Nodule occupation
Soybean
Strain selection
description A Bradyrhizobium japonicum USDA 110-derived strain able to produce wider halos in soft-agar medium than its parental strain was obtained by recurrent selection. It was more chemotactic than the wild type towards mannitol and three amino acids. When cultured in minimal medium with mannitol as a single carbon-source, it had one thick subpolar flagellum as the wild type, plus several other flagella that were thinner and sinusoidal. Root adsorption and infectivity in liquid media were 50-100% higher for the selected strain, but root colonization in water-unsaturated vermiculite was similar to the wild type. A field experiment was then carried out in a soil with a naturalized population of 1.8 × 105 soybean-nodulating rhizobia g of soil -1. Bradyrhizobium japonicum strains were inoculated either on the soybean seeds or in the sowing furrows. Nodule occupation was doubled when the strains were inoculated in the sowing furrows with respect to seed inoculation (significant with P<0.05). On comparing strains, nodule occupation with seed inoculation was 6% or 10% for the wild type or selected strains, respectively, without a statistically significant difference, while when inoculated in the sowing furrows, nodule occupation increased to 12% and 22%, respectively (differences significant with P<0.05).
format Articulo
Articulo
author Althabegoiti, María Julia
López García, Silvina Laura
Piccinetti, Carlos
Mongiardini, Elías Javier
Pérez Giménez, Julieta
Quelas, Juan Ignacio
Perticari, Alejandro
Lodeiro, Aníbal Roberto
author_facet Althabegoiti, María Julia
López García, Silvina Laura
Piccinetti, Carlos
Mongiardini, Elías Javier
Pérez Giménez, Julieta
Quelas, Juan Ignacio
Perticari, Alejandro
Lodeiro, Aníbal Roberto
author_sort Althabegoiti, María Julia
title Strain selection for improvement of Bradyrhizobium japonicum competitiveness for nodulation of soybean
title_short Strain selection for improvement of Bradyrhizobium japonicum competitiveness for nodulation of soybean
title_full Strain selection for improvement of Bradyrhizobium japonicum competitiveness for nodulation of soybean
title_fullStr Strain selection for improvement of Bradyrhizobium japonicum competitiveness for nodulation of soybean
title_full_unstemmed Strain selection for improvement of Bradyrhizobium japonicum competitiveness for nodulation of soybean
title_sort strain selection for improvement of bradyrhizobium japonicum competitiveness for nodulation of soybean
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/84576
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