Neonatal thymulin gene therapy prevents ovarian dysgenesis and attenuates reproductive derangements in nude female mice

Congenitally athymic (nude) female mice show severe ovarian dysgenesis after puberty, which seems to be consequential to a number of neuroendocrine derangements described in these mutants. Thus, considerable evidence suggests that thymulin, a thymic peptide, may be involved in thymus-pituitary commu...

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Autores principales:	Reggiani, Paula Cecilia, Barbeito, Claudio Gustavo, Zuccolilli, Gustavo Oscar, Cónsole-Avegliano, Gloria Miriam, Flamini, Alicia M., Dardenne, Mireille, Goya, Rodolfo Gustavo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2012
Materias:	Bioquímica neonatal thymulin gene therapy thymus-hypothalamo-pituitary- gonadal axis
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/84575
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-84575
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Bioquímica neonatal thymulin gene therapy thymus-hypothalamo-pituitary- gonadal axis
spellingShingle	Bioquímica neonatal thymulin gene therapy thymus-hypothalamo-pituitary- gonadal axis Reggiani, Paula Cecilia Barbeito, Claudio Gustavo Zuccolilli, Gustavo Oscar Cónsole-Avegliano, Gloria Miriam Flamini, Alicia M. Dardenne, Mireille Goya, Rodolfo Gustavo Neonatal thymulin gene therapy prevents ovarian dysgenesis and attenuates reproductive derangements in nude female mice
topic_facet	Bioquímica neonatal thymulin gene therapy thymus-hypothalamo-pituitary- gonadal axis
description	Congenitally athymic (nude) female mice show severe ovarian dysgenesis after puberty, which seems to be consequential to a number of neuroendocrine derangements described in these mutants. Thus, considerable evidence suggests that thymulin, a thymic peptide, may be involved in thymus-pituitary communication. In order to clarify the relevance of thymulin for the maturation of the female reproductive system, we assessed at hypothalamic, pituitary, ovarian, and uterine level the preventive action of neonatal thymulin gene therapy (NTGT) on the changes that typically occur after puberty in congenitally athymic female mice. We injected (im) an adenoviral vector harboring a synthetic DNA sequence encoding a biologically active analog of thymulin, methionine-serum thymic factor, in newborn nude mice (which are thymulin deficient) and killed the animals at 70-71 d of age. NTGT in the athymic mice restored the serum thymulin levels. Morphometric analysis revealed that athymic nudes have reduced numbers of brain GnRH neurons and pituitary gonadotropic cells as compared with heterozygous controls. NTGT prevented these changes and also rescued the premature ovarian failure phenotype typically observed in athymic nude mice (marked reduction in the number of antral follicles and corpora lutea, increase in atretic follicles). Serum estrogen, but not progesterone, levels were low in athymic nudes, a reduction that was partially prevented by NTGT. Little to no morphological changes were observed in the endometrium of female nudes. The delay in the age of vaginal opening that occurs in athymic nudes was significantly prevented by NTGT. Our results suggest that thymulin plays a relevant physiologic role in the thymus-hypothalamo-pituitary- gonadal axis.
format	Articulo Articulo
author	Reggiani, Paula Cecilia Barbeito, Claudio Gustavo Zuccolilli, Gustavo Oscar Cónsole-Avegliano, Gloria Miriam Flamini, Alicia M. Dardenne, Mireille Goya, Rodolfo Gustavo
author_facet	Reggiani, Paula Cecilia Barbeito, Claudio Gustavo Zuccolilli, Gustavo Oscar Cónsole-Avegliano, Gloria Miriam Flamini, Alicia M. Dardenne, Mireille Goya, Rodolfo Gustavo
author_sort	Reggiani, Paula Cecilia
title	Neonatal thymulin gene therapy prevents ovarian dysgenesis and attenuates reproductive derangements in nude female mice
title_short	Neonatal thymulin gene therapy prevents ovarian dysgenesis and attenuates reproductive derangements in nude female mice
title_full	Neonatal thymulin gene therapy prevents ovarian dysgenesis and attenuates reproductive derangements in nude female mice
title_fullStr	Neonatal thymulin gene therapy prevents ovarian dysgenesis and attenuates reproductive derangements in nude female mice
title_full_unstemmed	Neonatal thymulin gene therapy prevents ovarian dysgenesis and attenuates reproductive derangements in nude female mice
title_sort	neonatal thymulin gene therapy prevents ovarian dysgenesis and attenuates reproductive derangements in nude female mice
publishDate	2012
url	http://sedici.unlp.edu.ar/handle/10915/84575
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Neonatal thymulin gene therapy prevents ovarian dysgenesis and attenuates reproductive derangements in nude female mice

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