Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity

In chloroplasts, stromal and thylakoid-bound ascorbate peroxidases (tAPX) play a major role in the removal of H<SUB>2</SUB>O<SUB>2</SUB> produced during photosynthesis. Here, we report that hexaploid wheat (<i>Triticum aestivum</i>) expresses three homeologous tAP...

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Autores principales: Danna, Cristian H., Bartoli, Carlos Guillermo, Sacco, Francisco, Ingala, Lorena R., Santa María, Guillermo E., Guiamet, Juan José, Ugalde, Rodolfo A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Ciencias Agrarias
Ciencias Exactas
ascorbate
H2O2
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84571
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84571
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Ciencias Exactas
ascorbate
H2O2
spellingShingle Ciencias Agrarias
Ciencias Exactas
ascorbate
H2O2
Danna, Cristian H.
Bartoli, Carlos Guillermo
Sacco, Francisco
Ingala, Lorena R.
Santa María, Guillermo E.
Guiamet, Juan José
Ugalde, Rodolfo A.
Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity
topic_facet Ciencias Agrarias
Ciencias Exactas
ascorbate
H2O2
description In chloroplasts, stromal and thylakoid-bound ascorbate peroxidases (tAPX) play a major role in the removal of H<SUB>2</SUB>O<SUB>2</SUB> produced during photosynthesis. Here, we report that hexaploid wheat (<i>Triticum aestivum</i>) expresses three homeologous tAPX genes (TaAPX-6A, TaAPX-6B, and TaAPX-6D) mapping on group-6 chromosomes. The tAPX activity of a mutant line lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
format Articulo
Articulo
author Danna, Cristian H.
Bartoli, Carlos Guillermo
Sacco, Francisco
Ingala, Lorena R.
Santa María, Guillermo E.
Guiamet, Juan José
Ugalde, Rodolfo A.
author_facet Danna, Cristian H.
Bartoli, Carlos Guillermo
Sacco, Francisco
Ingala, Lorena R.
Santa María, Guillermo E.
Guiamet, Juan José
Ugalde, Rodolfo A.
author_sort Danna, Cristian H.
title Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity
title_short Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity
title_full Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity
title_fullStr Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity
title_full_unstemmed Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity
title_sort thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/84571
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