Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
In the present study, the role of direct procaryote-eucaryote interactions in the virulence of <i>Bacillus cereus</i> was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with <i>B. cereus</i>...
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| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2004
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/84452 |
| Aporte de: |
| Sumario: | In the present study, the role of direct procaryote-eucaryote interactions in the virulence of <i>Bacillus cereus</i> was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with <i>B. cereus</i> in the exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, addition of 3-h-old cultures of some strains resulted in complete detachment of the cultured cells after a 3-h infection whereas no such effect was found after a 3-h infection with 16-h-old cultures. Infection of enterocyte-like cells with <i>B. cereus</i> leads to disruption of the F-actin network and necrosis. Even though the effect of secreted factors cannot be ruled out, direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some <i>B. cereus</i> strains were able to be internalized in Caco-2 cells. Our findings add a new insight into the mechanisms of virulence of <i>B. cereus</i> in the context of intestinal infection. |
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