Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells

In the present study, the role of direct procaryote-eucaryote interactions in the virulence of <i>Bacillus cereus</i> was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with <i>B. cereus</i>...

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Detalles Bibliográficos
Autores principales: Minnaard, Jessica, Lievin Le Moal, Vanessa, Coconnier, Marie Helene, Servin, Alain L., Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2004
Materias:
Ciencias Exactas
Bacillus cereus
intestinal infection
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84452
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84452
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bacillus cereus
intestinal infection
spellingShingle Ciencias Exactas
Bacillus cereus
intestinal infection
Minnaard, Jessica
Lievin Le Moal, Vanessa
Coconnier, Marie Helene
Servin, Alain L.
Pérez, Pablo Fernando
Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
topic_facet Ciencias Exactas
Bacillus cereus
intestinal infection
description In the present study, the role of direct procaryote-eucaryote interactions in the virulence of <i>Bacillus cereus</i> was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with <i>B. cereus</i> in the exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, addition of 3-h-old cultures of some strains resulted in complete detachment of the cultured cells after a 3-h infection whereas no such effect was found after a 3-h infection with 16-h-old cultures. Infection of enterocyte-like cells with <i>B. cereus</i> leads to disruption of the F-actin network and necrosis. Even though the effect of secreted factors cannot be ruled out, direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some <i>B. cereus</i> strains were able to be internalized in Caco-2 cells. Our findings add a new insight into the mechanisms of virulence of <i>B. cereus</i> in the context of intestinal infection.
format Articulo
Articulo
author Minnaard, Jessica
Lievin Le Moal, Vanessa
Coconnier, Marie Helene
Servin, Alain L.
Pérez, Pablo Fernando
author_facet Minnaard, Jessica
Lievin Le Moal, Vanessa
Coconnier, Marie Helene
Servin, Alain L.
Pérez, Pablo Fernando
author_sort Minnaard, Jessica
title Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_short Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_full Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_fullStr Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_full_unstemmed Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_sort disassembly of f-actin cytoskeleton after interaction of bacillus cereus with fully differentiated human intestinal caco-2 cells
publishDate 2004
url http://sedici.unlp.edu.ar/handle/10915/84452
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AT lievinlemoalvanessa disassemblyoffactincytoskeletonafterinteractionofbacilluscereuswithfullydifferentiatedhumanintestinalcaco2cells
AT coconniermariehelene disassemblyoffactincytoskeletonafterinteractionofbacilluscereuswithfullydifferentiatedhumanintestinalcaco2cells
AT servinalainl disassemblyoffactincytoskeletonafterinteractionofbacilluscereuswithfullydifferentiatedhumanintestinalcaco2cells
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bdutipo_str Repositorios
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