Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells

In the present study, the role of direct procaryote-eucaryote interactions in the virulence of <i>Bacillus cereus</i> was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with <i>B. cereus</i>...

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Detalles Bibliográficos
Autores principales:	Minnaard, Jessica, Lievin Le Moal, Vanessa, Coconnier, Marie Helene, Servin, Alain L., Pérez, Pablo Fernando
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2004
Materias:	Ciencias Exactas Bacillus cereus intestinal infection
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/84452
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-84452
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Bacillus cereus intestinal infection
spellingShingle	Ciencias Exactas Bacillus cereus intestinal infection Minnaard, Jessica Lievin Le Moal, Vanessa Coconnier, Marie Helene Servin, Alain L. Pérez, Pablo Fernando Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
topic_facet	Ciencias Exactas Bacillus cereus intestinal infection
description	In the present study, the role of direct procaryote-eucaryote interactions in the virulence of <i>Bacillus cereus</i> was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with <i>B. cereus</i> in the exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, addition of 3-h-old cultures of some strains resulted in complete detachment of the cultured cells after a 3-h infection whereas no such effect was found after a 3-h infection with 16-h-old cultures. Infection of enterocyte-like cells with <i>B. cereus</i> leads to disruption of the F-actin network and necrosis. Even though the effect of secreted factors cannot be ruled out, direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some <i>B. cereus</i> strains were able to be internalized in Caco-2 cells. Our findings add a new insight into the mechanisms of virulence of <i>B. cereus</i> in the context of intestinal infection.
format	Articulo Articulo
author	Minnaard, Jessica Lievin Le Moal, Vanessa Coconnier, Marie Helene Servin, Alain L. Pérez, Pablo Fernando
author_facet	Minnaard, Jessica Lievin Le Moal, Vanessa Coconnier, Marie Helene Servin, Alain L. Pérez, Pablo Fernando
author_sort	Minnaard, Jessica
title	Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_short	Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_full	Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_fullStr	Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_full_unstemmed	Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells
title_sort	disassembly of f-actin cytoskeleton after interaction of bacillus cereus with fully differentiated human intestinal caco-2 cells
publishDate	2004
url	http://sedici.unlp.edu.ar/handle/10915/84452
work_keys_str_mv	AT minnaardjessica disassemblyoffactincytoskeletonafterinteractionofbacilluscereuswithfullydifferentiatedhumanintestinalcaco2cells AT lievinlemoalvanessa disassemblyoffactincytoskeletonafterinteractionofbacilluscereuswithfullydifferentiatedhumanintestinalcaco2cells AT coconniermariehelene disassemblyoffactincytoskeletonafterinteractionofbacilluscereuswithfullydifferentiatedhumanintestinalcaco2cells AT servinalainl disassemblyoffactincytoskeletonafterinteractionofbacilluscereuswithfullydifferentiatedhumanintestinalcaco2cells AT perezpablofernando disassemblyoffactincytoskeletonafterinteractionofbacilluscereuswithfullydifferentiatedhumanintestinalcaco2cells
bdutipo_str	Repositorios
_version_	1764820488752726016

Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells

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