Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes

Thyroid hormones are important regulators of lipid metabolism. Polymorphonuclear leukocytes (PMN) are essential components of innate immune response. Our goal was to determine whether hypothyroidism affects lipid metabolism in PMN cells. Wistar rats were made hypothyroid by administrating 0.1 g/L 6-...

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Detalles Bibliográficos
Autores principales:	Coria, Mariela J., Carmona Viglianco, Yamila V., Marra, Carlos Alberto, Gomez-Mejiba, Sandra E., Ramirez, Dario C., Anzulovich, Ana C., Gimenez, Maria S.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2012
Materias:	Ciencias Médicas Cholesterol HMGCoAR Hypothyroidism Triacylglycerides
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/84430
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-84430
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas Cholesterol HMGCoAR Hypothyroidism Triacylglycerides
spellingShingle	Ciencias Médicas Cholesterol HMGCoAR Hypothyroidism Triacylglycerides Coria, Mariela J. Carmona Viglianco, Yamila V. Marra, Carlos Alberto Gomez-Mejiba, Sandra E. Ramirez, Dario C. Anzulovich, Ana C. Gimenez, Maria S. Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes
topic_facet	Ciencias Médicas Cholesterol HMGCoAR Hypothyroidism Triacylglycerides
description	Thyroid hormones are important regulators of lipid metabolism. Polymorphonuclear leukocytes (PMN) are essential components of innate immune response. Our goal was to determine whether hypothyroidism affects lipid metabolism in PMN cells. Wistar rats were made hypothyroid by administrating 0.1 g/L 6-propyl-2-thiouracil (PTU) in drinking water during 30 days. Triacylglycerides (TG), cholesterol and phospholipids were determined in PMN and serum by conventional methods. The mRNA expression of LDL receptor (LDL-R), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCoAR), sterol regulatory element binding protein 2 (SREBP-2), and diacylglycerol acyltransferase 2 (DGAT-2) were quantified by Real-Time PCR. Cellular neutral lipids were identified by Nile red staining. We found hypothyroidism decreases serum TG whereas it increases them in PMN. This result agrees with those observed in Nile red preparations, however DAGT-2 expression was not modified. Cholesterol synthesizing enzyme HMGCoAR mRNA and protein was reduced in PMN of hypothyroid rats. As expected, cholesterol content decreased in the cells although it increased in serum. Hypothyroidism also reduced relative contents of palmitic, stearic, and arachidonic acids, whereas increased the myristic, linoleic acids, and the unsaturation index in PMN. Thus, hypothyroidism modifies PMN lipid composition. These findings would emphasize the importance of new research to elucidate lipid-induced alterations in specific function(s) of PMN.
format	Articulo Articulo
author	Coria, Mariela J. Carmona Viglianco, Yamila V. Marra, Carlos Alberto Gomez-Mejiba, Sandra E. Ramirez, Dario C. Anzulovich, Ana C. Gimenez, Maria S.
author_facet	Coria, Mariela J. Carmona Viglianco, Yamila V. Marra, Carlos Alberto Gomez-Mejiba, Sandra E. Ramirez, Dario C. Anzulovich, Ana C. Gimenez, Maria S.
author_sort	Coria, Mariela J.
title	Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes
title_short	Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes
title_full	Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes
title_fullStr	Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes
title_full_unstemmed	Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes
title_sort	hypothyroidism modifies lipid composition of polymorphonuclear leukocytes
publishDate	2012
url	http://sedici.unlp.edu.ar/handle/10915/84430
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bdutipo_str	Repositorios
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Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes

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